| MWexperimental | 16 | kDa |
| MWexpected | 22 | kDa |
| VPorod | 25 | nm3 |
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log I(s)
6.85×10-1
6.85×10-2
6.85×10-3
6.85×10-4
|
s, nm-1
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Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences.
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO, Nucleic Acids Res (2022) Europe PMCSASDM36 – c-Myc12 DnaseI Treated G-quadruplex
c-Myc 12-tract G-quadruplex monomer Dnase I treated|
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Fits and models
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Synchrotron SAXS
data from solutions of
c-Myc12 DnaseI Treated G-quadruplex
in
6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a sample-detector distance of 3.5 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 270.00 μl sample
at 2.3 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 75 Increase 10/300 column
at 25°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
C-Myc 12-G-tract promoter region annealed in potassium phosphate buffer prior to digestion with DNase I to remove duplex or single-stranded DNA features. |
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