Synchrotron SAXS data from solutions of the hunchback mRNA translation repression complex (Brat-NHL, Pum-HD, Nanos-ZnF and hb NRE2 RNA) in 50 mM Tris, 150 mM NaCl, 1 mM DTT, 3% glycerol, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 75.00 μl sample at 7.2 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the appropriate SEC peak data frames through the elution profile of the complex.
s, nm-1
