Search

  
Advanced search  

6 hits found for mRNA endoribonuclease toxin LS

SASDHW7 – ...toxin-antitoxin complex (mRNA endoribonuclease toxin LS : Antitoxin RnlB)

mRNA endoribonuclease toxin LSAntitoxin RnlB (C-terminal His-tagged) experimental SAS data
mRNA endoribonuclease toxin LS Antitoxin RnlB (C-terminal His-tagged) Kratky plot
Sample: mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
...toxin RnlB (C-terminal His-tagged) monomer, 15 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...ls J, Loris R
RgGuinier 3.5 nm
Dmax 10.0 nm
VolumePorod 161 nm3

SASDHX7 – ...mRNA endoribonuclease toxin LS)

mRNA endoribonuclease toxin LS experimental SAS data
mRNA endoribonuclease toxin LS Kratky plot
Sample: mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...ls J, Loris R
RgGuinier 3.7 nm
Dmax 10.7 nm
VolumePorod 134 nm3

SASDKL2 – ...mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)

NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data
MULTIFOXS model
Sample: ...endoribonuclease dimer, 62 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jun 27
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...ls J, Loris R
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 88 nm3

SASDHY7 – ...mRNA endoribonuclease toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged experimental SAS data
mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot
Sample: mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...ls J, Loris R
RgGuinier 3.7 nm
Dmax 20.1 nm
VolumePorod 133 nm3

SASDKS9 – mRNA endoribonuclease toxin LS (D245R mutant); batch-SAXS measurements in low salt buffer

mRNA endoribonuclease toxin LS (D245R mutant) experimental SAS data
MULTIFOXS model
Sample: mRNA endoribonuclease toxin LS (D245R mutant) dimer, 84 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...ls J, Loris R
RgGuinier 4.3 nm
Dmax 15.9 nm
VolumePorod 183 nm3

SASDHZ7 – ...mRNA endoribonuclease toxin LS) - R318A single alanine mutant

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged experimental SAS data
mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged Kratky plot
Sample: mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...ls J, Loris R
RgGuinier 3.4 nm
Dmax 12.7 nm
VolumePorod 124 nm3