A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR).

Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M, J Biol Chem 287(41):34304-15 (2012) Europe PMC

SASDAV4 – uPAR wild-type AE105 complex

Urokinase plasminogen activator surface receptor
synthetic peptide AE105
MWI(0) 37 kDa
MWexpected 38 kDa
VPorod 61 nm3
log I(s) 3.92×101 3.92×100 3.92×10-1 3.92×10-2
Urokinase plasminogen activator surface receptor synthetic peptide AE105 small angle scattering data  s, nm-1
ln I(s)
Urokinase plasminogen activator surface receptor synthetic peptide AE105 Guinier plot ln 3.92×101 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Urokinase plasminogen activator surface receptor synthetic peptide AE105 Kratky plot 1.104 0 3 sRg
p(r)
Urokinase plasminogen activator surface receptor synthetic peptide AE105 pair distance distribution function Rg: 2.5 nm 0 Dmax: 8.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of uPAR wild-type AE105 complex in 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH 7.2 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.5 and 4.4 mg/ml were measured at 10°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Urokinase plasminogen activator surface receptor (uPAR)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   37.0 kDa
 
UniProt   Q03405
Sequence   FASTA
 
synthetic peptide AE105 (AE105)
Mol. type   Protein
Olig. state   Monomer
Mon. MW   1.2 kDa
Sequence   FASTA