Structure and dynamics of the von Willebrand Factor C6 domain

SASDLS9 – von Willebrand factor C6-CK wildtype

von Willebrand factor C6-CK wildtype
MWexperimental 46 kDa
MWexpected 41 kDa
VPorod 90 nm3
log I(s) 1.94×10-2 1.94×10-3 1.94×10-4 1.94×10-5
von Willebrand factor C6-CK wildtype small angle scattering data  s, nm-1
ln I(s)
von Willebrand factor C6-CK wildtype Guinier plot ln 1.94×10-2 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
von Willebrand factor C6-CK wildtype Kratky plot 1.104 0 √3 sRg
p(r)
von Willebrand factor C6-CK wildtype pair distance distribution function Rg: 3.5 nm 0 Dmax: 15 nm

Data validation

There are no models related to this curve.

ynchrotron SAXS data from solutions of von Willebrand factor C6-CK, wildtype, in 20 mM HEPES, 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 25 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

von Willebrand factor C6-CK wildtype (VWFC6-CK wt)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   20.7 kDa
 
UniProt   P04275 (2647-2813)
Sequence   FASTA