Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: other species

SASDXJ2 – Mammalian translation elongation factor eEF1A1

Mammalian translation elongation factor eEF1A1 experimental SAS data

Sample:	Mammalian translation elongation factor eEF1A1 dimer, 100 kDa protein
Buffer:	25 mM Tris HCl, 150 mM NaCl, 6 mM βME, 20% glycerol, 0.01mM GDP,, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar w Excillum source, Department of Chemistry, iNANO building, Aarhus Uinversity on 2023 Mar 20

Dynamics and structural features of the eEF1A1 and eEF1A2 paralogs Nucleic Acids Research 53(21) (2025)
Novosylna O, Shalak V, Dąbrowska K, Patmanidis I, Lozhko D, Bondarchuk T, Schiøtt B, Pedersen J, Knudsen C, Nissen P, Dadlez M, Negrutskii B

R_g^Guinier	4.6	nm
D_max	143.1	nm
Volume^Porod	144	nm³

SASDYJ2 – Hen Egg White Lysozyme pH 7.4

Hen egg white lysozyme experimental SAS data

Sample:	Hen egg white lysozyme monomer, 14 kDa Gallus gallus protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 9

Lysozyme pH 7.4 with models
Anthony Giannetti

R_g^Guinier	1.6	nm
D_max	59.0	nm
Volume^Porod	23	nm³

SASDXB7 – Entamoeba histolytica Anti-Silencing Function 1 (ASF1) with C terminal His6-tag

Anti-Silencing Function 1 experimental SAS data

Sample:	Anti-Silencing Function 1 monomer, 28 kDa Entamoeba histolytica strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2023 Oct 1

Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone. Biochimie (2025)
Gandhi S, Vasudevan D

R_g^Guinier	3.5	nm
D_max	10.1	nm
Volume^Porod	40	nm³

SASDXC7 – Entamoeba histolytica Anti-Silencing Function 1 (ASF1)_Histone H3-H4 dimer complex

Anti-Silencing Function 1Histone H3Histone H4 experimental SAS data

Sample:	Anti-Silencing Function 1 monomer, 28 kDa Entamoeba histolytica strain … protein Histone H3 monomer, 15 kDa Entamoeba histolytica strain … protein Histone H4 monomer, 13 kDa Entamoeba histolytica strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, and 2 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2023 Oct 1

Characterisation of Entamoeba histolytica Anti-Silencing Function 1 as a histone chaperone. Biochimie (2025)
Gandhi S, Vasudevan D

R_g^Guinier	2.9	nm
D_max	8.0	nm
Volume^Porod	84	nm³

SASDWN5 – N-acetylmuramoyl-L-alanine amidase (without His-Tag)

N-acetylmuramoyl-L-alanine amidase experimental SAS data

Sample:	N-acetylmuramoyl-L-alanine amidase monomer, 43 kDa Mycobacterium phage DS6A protein
Buffer:	20 mM Tris, 150 mM NaCl and 1 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2024 Sep 30

Dissecting the molecular basis underlying mycobacterial cell-wall hydrolysis by the catalytic domains of D29LysA and DS6ALysA phage endolysins. Int J Biol Macromol :148896 (2025)
Ceballos-Zúñiga F, Galvez-Larrosa L, Muñoz IG, Infantes L, Fernández-Carrillo J, Pérez-Dorado I

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	50	nm³

SASDWP5 – N-acetylmuramoyl-L-alanine amidase (with His-Tag)

N-acetylmuramoyl-L-alanine amidase (Mycobacteriophage DS6A) experimental SAS data

Sample:	N-acetylmuramoyl-L-alanine amidase (Mycobacteriophage DS6A) monomer, 45 kDa protein
Buffer:	20 mM Tris, 150 mM NaCl and 1 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Mar 30

R_g^Guinier	2.8	nm
D_max	10.3	nm
Volume^Porod	62	nm³

SASDXF6 – RTX cytotoxin pro-MbxA without acylation (pro-MbxA)

RTX cytotoxin pro-MbxA without acylation experimental SAS data

Sample:	RTX cytotoxin pro-MbxA without acylation monomer, 100 kDa Moraxella bovis protein
Buffer:	50 mM Tris, 100 mM NaCl, 10mM CaCl2, pH: 7.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 May 9

Acylation of the RTX toxin MbxA stimulates host membrane disruption through a specific interaction with cholesterol Biochimica et Biophysica Acta (BBA) - Biomembranes :184487 (2025)
Chacko F, Ganz S, Pfitzer-Bilsing A, Hänsch S, Westhoff P, Weidtkamp-Peters S, Smits S, Exterkate M, Schmitt L

R_g^Guinier	3.8	nm
D_max	13.4	nm
Volume^Porod	154	nm³

SASDXP4 – Mutual gliding motility protein C from Myxococcus xanthus

Mutual gliding motility protein C experimental SAS data

Sample:	Mutual gliding motility protein C dimer, 32 kDa Myxococcus xanthus (strain … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Mar 31

Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus Journal of Biological Chemistry :110907 (2025)
Kodesia A, Kapoor S, Thakur K

R_g^Guinier	2.3	nm
D_max	9.8	nm
Volume^Porod	49	nm³

SASDXQ4 – C-terminal helix of RomR protein from Myxococcus xanthus

Two-component system response regulator experimental SAS data

Sample:	Two-component system response regulator trimer, 26 kDa Myxococcus xanthus protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Mar 31

R_g^Guinier	2.7	nm
D_max	10.3	nm
Volume^Porod	43	nm³

SASDXR4 – C-terminal region of RomR protein from Myxococcus xanthus

Two-component system response regulator Kratky plot

Sample:	Two-component system response regulator trimer, 39 kDa Myxococcus xanthus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Mar 31

R_g^Guinier	4.1	nm
D_max	16.7	nm
Volume^Porod	81	nm³

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