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SASDN92 – Apoform of glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p)

Glyceraldehyde-3-phosphate dehydrogenase 1 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glyceraldehyde-3-phosphate dehydrogenase 1 tetramer, 142 kDa Kluyveromyces marxianus protein
Buffer: 150 mM NaCl, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10 mM TrisHCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Mar 27
The Crystal and Solution Structures of Glyceraldehyde-3-phosphate Dehydrogenase Reveal Different Quaternary Structures Journal of Biological Chemistry 281(44):33433-33440 (2006)
Ferreira-da-Silva F, Pereira P, Gales L, Roessle M, Svergun D, Moradas-Ferreira P, Damas A
RgGuinier 4.2 nm
Dmax 12.0 nm
VolumePorod 234 nm3

SASDNA2 – Glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p) upon NAD+ binding

Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ tetramer, 142 kDa Kluyveromyces marxianus protein
Buffer: 150 mM NaCl, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10 mM TrisHCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Mar 27
The Crystal and Solution Structures of Glyceraldehyde-3-phosphate Dehydrogenase Reveal Different Quaternary Structures Journal of Biological Chemistry 281(44):33433-33440 (2006)
Ferreira-da-Silva F, Pereira P, Gales L, Roessle M, Svergun D, Moradas-Ferreira P, Damas A
RgGuinier 3.7 nm
Dmax 9.9 nm
VolumePorod 202 nm3

SASDAQ5 – Lumazine Synthase

Lumazine Synthase experimental SAS data
DAMMIN model
Sample: Lumazine Synthase, 960 kDa Bacillus subtilis protein
Buffer: Borate buffer, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Nov 25
Multiple assembly states of lumazine synthase: a model relating catalytic function and molecular assembly. J Mol Biol 362(4):753-70 (2006)
Zhang X, Konarev PV, Petoukhov MV, Svergun DI, Xing L, Cheng RH, Haase I, Fischer M, Bacher A, Ladenstein R, Meining W
RgGuinier 6.2 nm
Dmax 15.5 nm
VolumePorod 1450 nm3

SASDAD4 – Full length GtBP3

full length CtBP3 experimental SAS data
DAMMIN model
Sample: Full length CtBP3 tetramer, 170 kDa protein
Buffer: 25 mM Tris/HCl 250 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Nov 18
The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured. Protein Sci 15(5):1042-50 (2006)
Nardini M, Svergun D, Konarev PV, Spanò S, Fasano M, Bracco C, Pesce A, Donadini A, Cericola C, Secundo F, Luini A, Corda D, Bolognesi M
RgGuinier 5.1 nm
Dmax 19.0 nm
VolumePorod 330 nm3

SASDAE4 – C-terminal CtBP3

C-term part CtBP3 experimental SAS data
DAMMIN model
Sample: C-term part CtBP3 dimer, 52 kDa protein
Buffer: 25 mM Tris/HCl 250 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Nov 18
The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured. Protein Sci 15(5):1042-50 (2006)
Nardini M, Svergun D, Konarev PV, Spanò S, Fasano M, Bracco C, Pesce A, Donadini A, Cericola C, Secundo F, Luini A, Corda D, Bolognesi M
RgGuinier 5.5 nm
Dmax 20.0 nm
VolumePorod 126 nm3

SASDLQ5 – Tricorn protease oligomers

Tricorn protease experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tricorn protease hexamer, 730 kDa Thermoplasma acidophilum (strain … protein
Buffer: 20mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Jan 14
X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum. J Biol Chem 280(39):33387-96 (2005)
Goettig P, Brandstetter H, Groll M, Göhring W, Konarev PV, Svergun DI, Huber R, Kim JS
RgGuinier 5.9 nm

SASDLN7 – 1:1 complex of Ferredoxin-dependent glutamate synthase 2 (FdGlts) with ferredoxin

Ferredoxin-dependent glutamate synthase 2Ferredoxin-1 experimental SAS data
OTHER model
Sample: Ferredoxin-dependent glutamate synthase 2 monomer, 169 kDa Synechocystis sp. (strain … protein
Ferredoxin-1 monomer, 11 kDa Nostoc sp. (strain … protein
Buffer: Hepes– KOH buffer, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2002 Jun 4
The Active Conformation of Glutamate Synthase and its Binding to Ferredoxin Journal of Molecular Biology 330(1):113-128 (2003)
van den Heuvel R, Svergun D, Petoukhov M, Coda A, Curti B, Ravasio S, Vanoni M, Mattevi A
RgGuinier 3.6 nm
Dmax 12.3 nm
VolumePorod 262 nm3

SASDAX2 – Pyruvate decarboxylase (PDC) from Z. mobilis

Pyruvate decarboxylase experimental SAS data
CRYSOL model
Sample: Pyruvate decarboxylase tetramer, 244 kDa Zymomonas mobilis protein
Buffer: 100 mM Sodium Citrate, 17% Glycerol, 22.5% PEG 1500, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 1998 Nov 3
Crystal versus solution structures of thiamine diphosphate-dependent enzymes. J Biol Chem 275(1):297-302 (2000)
Svergun DI, Petoukhov MV, Koch MH, König S
RgGuinier 3.9 nm
Dmax 11.0 nm

SASDUS6 – Amino-terminal domain of Infectious Bursal Disease Virus VP3 at pH8.0

Structural polyprotein experimental SAS data
Structural polyprotein Kratky plot
Sample: Structural polyprotein dimer, 26 kDa Infectious bursal disease … protein
Buffer: 50 mM Tris, 500 mM NaCl, 2 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 27
Infectious Bursal Disease Virus VP3
Diego Ferrero
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 52 nm3

SASDUR7 – Infectious Bursal Disease Virus VP3 (central domains at pH 8.0)

Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) experimental SAS data
Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) Kratky plot
Sample: Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) monomer, 18 kDa Infectious bursal disease … protein
Buffer: 50 mM TRIS, 500 mM NaCl, 2 mM DTT,, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
Infectious Bursal Disease Virus VP3
Diego Ferrero
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 21 nm3