|
|
|
|
|
| Sample: |
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
|
| Buffer: |
20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
|
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Mar 17
|
Ligand‐induced CaMKIIα
hub Trp403 flip, hub domain stacking, and modulation of kinase activity
Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
|
| RgGuinier |
4.7 |
nm |
| Dmax |
16.5 |
nm |
| VolumePorod |
358 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
|
| Buffer: |
20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
|
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2023 Mar 17
|
Ligand‐induced CaMKIIα
hub Trp403 flip, hub domain stacking, and modulation of kinase activity
Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
|
| RgGuinier |
4.4 |
nm |
| Dmax |
15.0 |
nm |
| VolumePorod |
334 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
|
| Buffer: |
20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
|
| Experiment: |
SAXS
data collected at Xenocs BioXolver L, Département de Biochimie, Université de Montréal on 2022 Dec 15
|
Ligand‐induced CaMKIIα
hub Trp403 flip, hub domain stacking, and modulation of kinase activity
Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
|
| RgGuinier |
4.4 |
nm |
| Dmax |
15.0 |
nm |
| VolumePorod |
335 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
|
| Buffer: |
20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
|
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2022 Oct 3
|
Ligand‐induced CaMKIIα
hub Trp403 flip, hub domain stacking, and modulation of kinase activity
Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
|
| RgGuinier |
4.7 |
nm |
| Dmax |
18.0 |
nm |
| VolumePorod |
329 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
|
| Buffer: |
20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
|
| Experiment: |
SAXS
data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
|
Ligand‐induced CaMKIIα
hub Trp403 flip, hub domain stacking, and modulation of kinase activity
Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
|
| RgGuinier |
4.5 |
nm |
| Dmax |
14.5 |
nm |
| VolumePorod |
313 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
|
| Buffer: |
20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
|
| Experiment: |
SAXS
data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30
|
Ligand‐induced CaMKIIα
hub Trp403 flip, hub domain stacking, and modulation of kinase activity
Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
|
| RgGuinier |
4.7 |
nm |
| Dmax |
20.0 |
nm |
| VolumePorod |
375 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
|
| Buffer: |
20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
|
| Experiment: |
SAXS
data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
|
Ligand‐induced CaMKIIα
hub Trp403 flip, hub domain stacking, and modulation of kinase activity
Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
|
| RgGuinier |
4.7 |
nm |
| Dmax |
18.5 |
nm |
| VolumePorod |
366 |
nm3 |
|
|
|
|
|
|
![OTHER [STATIC IMAGE] model](/media/pdb_file/SASDSJ5_fit1_model1.png)
|
| Sample: |
15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct
|
| Buffer: |
100 mM NaCl, 20 mM Tris-HCl, pH: 7.4
|
| Experiment: |
SAXS
data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21
|
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose).
Nat Commun 15(1):7901 (2024)
Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
|
| RgGuinier |
2.4 |
nm |
| Dmax |
10.0 |
nm |
| VolumePorod |
15 |
nm3 |
|
|
|
|
|
|
![OTHER [STATIC IMAGE] model](/media/pdb_file/SASDSK5_fit1_model1.png)
|
| Sample: |
15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct
|
| Buffer: |
20 mM Tris-HCl, 1 mM MgCl2, 100 mM NaCl, pH: 7.4
|
| Experiment: |
SAXS
data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21
|
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose).
Nat Commun 15(1):7901 (2024)
Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
|
| RgGuinier |
2.3 |
nm |
| Dmax |
9.0 |
nm |
| VolumePorod |
14 |
nm3 |
|
|
|
|
|
|
![OTHER [STATIC IMAGE] model](/media/pdb_file/SASDSL5_fit1_model1.png)
|
| Sample: |
22mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 12 kDa synthetic construct
|
| Buffer: |
100 mM NaCl, 20 mM Tris-HCl, pH: 7.4
|
| Experiment: |
SAXS
data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Apr 21
|
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose).
Nat Commun 15(1):7901 (2024)
Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
|
| RgGuinier |
3.3 |
nm |
| Dmax |
14.0 |
nm |
| VolumePorod |
32 |
nm3 |
|
|
![Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data](/media/intensities_files/scattering_plots/SASDU25_dat_img.png "Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data")
![Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid Kratky plot](/media/intensities_files/scattering_plots/SASDU25_kratky_img.png)
![Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data](/media/intensities_files/scattering_plots/SASDU65_dat_img.png "Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data")
![Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid Kratky plot](/media/intensities_files/scattering_plots/SASDU65_kratky_img.png)
