SASDQU8 – Light-state LOV-activated diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data
DAMMIN model
Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, 3% glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2022 Apr 24
LOV-activated diguanylate cyclase
RgGuinier 5.2 nm
Dmax 18.0 nm
VolumePorod 159 nm3

SASDSK2 – SEC-SAXS data of wild-type estrogen receptor alpha, N-terminal domain (NTD) at pH 7.4

Estrogen receptor experimental SAS data
Estrogen receptor Kratky plot
Sample: Estrogen receptor monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 150 mM NaCl, 0.5 mM EDTA, 0.1 mM PMSF, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jul 21
SEC-SAXS profiles of ERalpha-NTD: wild-type, mutant, and phosphorylated Ser118 variants
Sichun Yang
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 47 nm3

SASDSE5 – SEC-SAXS data of mutant estrogen receptor alpha (S118A), N-terminal domain (NTD) at pH 7.4

Estrogen receptor (mutant S118A) experimental SAS data
Estrogen receptor (mutant S118A) Kratky plot
Sample: Estrogen receptor (mutant S118A) monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 150 mM NaCl, 0.5 mM EDTA, 0.1 mM PMSF, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jul 21
SEC-SAXS profiles of ERalpha-NTD: wild-type, mutant, and phosphorylated Ser118 variants
Sichun Yang
RgGuinier 3.2 nm
Dmax 15.0 nm
VolumePorod 71 nm3

SASDSF5 – SEC-SAXS data of mutant estrogen receptor alpha (S118D), N-terminal domain (NTD) at pH 7.4

Estrogen receptor (mutant S118D) experimental SAS data
Estrogen receptor (mutant S118D) Kratky plot
Sample: Estrogen receptor (mutant S118D) monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 150 mM NaCl, 0.5 mM EDTA, 0.1 mM PMSF, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Jul 21
SEC-SAXS profiles of ERalpha-NTD: wild-type, mutant, and phosphorylated Ser118 variants
Sichun Yang
RgGuinier 3.5 nm
Dmax 16.0 nm
VolumePorod 62 nm3

SASDVC5 – SEC-SAXS data of phosphorylated estrogen receptor alpha (pSer118), N-terminal domain (NTD) at pH 7.4

Estrogen receptor experimental SAS data
Estrogen receptor Kratky plot
Sample: Estrogen receptor monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jul 17
SEC-SAXS profiles of ERalpha-NTD: wild-type, mutant, and phosphorylated Ser118 variants
Sichun Yang
RgGuinier 3.6 nm
Dmax 16.0 nm
VolumePorod 70 nm3

SASDQV8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-free (Apo) state

Na/Ca-exchange protein, isoform D experimental SAS data
Na/Ca-exchange protein, isoform D Kratky plot
Sample: Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 2 mM EDTA, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
SAXS characterisation of CALX1.2 CBD12 construct
Roberto Kopke Salinas
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 48 nm3

SASDQW8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-bound state

Na/Ca-exchange protein, isoform D experimental SAS data
Na/Ca-exchange protein, isoform D Kratky plot
Sample: Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 50 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
SAXS characterisation of CALX1.2 CBD12 construct
RgGuinier 2.7 nm
Dmax 9.9 nm
VolumePorod 32 nm3

SASDUL9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 11.6 nm
VolumePorod 23 nm3

SASDUM9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 13.8 nm
VolumePorod 18 nm3

SASDUN9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 29
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.9 nm
VolumePorod 23 nm3

4417 hits found.