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Synchrotron SAXS
data from solutions of
Lysozyme amyloid fibril (LAF)
in
0.2 M glycine-HCl, 80 mM NaCl, pH 2.2
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 4.2 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 20°C.
20 successive
0.100 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
HEWL powder was dissolved in 0.2 M glycine-hydrochloric acid buffer (pH = 2.2) to form amyloid fibril solutions. These basic solutions were addedwith 80 mM NaCl. To induce the process of fibrillization, the prepared solutions were heated for 2 hours at 65 °C under constant stirring of 1200 rounds per minute.
In air SAXS measurement. Temperature controlled capillary holder.
The Guinier range of the SAXS profile cannot be resolved within the angular range at E = 10 keV and sample-detector distance of 4.17 m.
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s, nm-1
