Jowsey WJ,
Morris CRP,
Hall DA,
Sullivan JT,
Fagerlund RD,
Eto KY,
Solomon PD,
Mackay JP,
Bond CS,
Ramsay JP,
Ronson CW,
Nucleic Acids Res
(2023)
Europe PMC
Synchrotron SAXS data from solutions of QseM in 100 mM NaCl, 50 mM Tris-HCl, 2% glycerol, pH 7.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.25 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 22°C. 28 successive 1 second frames were collected through the SEC elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Monomeric triple-helix protein that binds and inhibits both TraR and FseA transcriptional regulator proteins from Mesorhizobium Loti R7A. The protein has a hexahistidine tag fused to the N-terminus for purification.
s, nm-1
