|
Synchrotron SAXS data from solutions of accumulation-association protein (Aap) Brpt5.5 2xH85A tetramer in the presence of ZnCl2 in 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 275.00 μl sample at 7.1 mg/ml was injected at a 0.80 ml/min flow rate onto a GE Superose 6 Increase 10/300 column at 23°C. 2843 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Brpt5.5 2xH85A (G5 08 - G5 13) in the presence of 5 mM ZnCl2, which induces tetramerization. This is a mutant construct which contains a His to Ala mutation in the 85 position of spacer subdomains 09 and 11 (H213A, H469A).
|
|
s, nm-1
