| MWexperimental | 66 | kDa |
| MWexpected | 68 | kDa |
| VPorod | 104 | nm3 |
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log I(s)
2.49×101
2.49×100
2.49×10-1
2.49×10-2
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s, nm-1
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7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system.
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D, Nucleic Acids Res (2023) Europe PMCSASDQ79 – Mutant 7-deazapurine in DNA protein A (D95A) bound to a 28 base pair DNA substrate (2-state model: complex plus free DNA)
Custom 28 base pair double stranded DNADNA-guanine transglycosylase - D95A mutant
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Fits and models
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Synchrotron SAXS data from solutions of mutant 7-deazapurine in DNA protein A (D95A) bound to a 28 base pair DNA in 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH 7 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 4 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex PROTEIN KW-802.5 size exclusion column (8.0 mm I.D. x 300 mm) at 24.9°C. Scattering images were collected with 3-s exposures per frame over the course of 40 minutes. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection volume = UNKNOWN |
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