Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors.

Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K, Protein J (2023) Europe PMC

SASDS45 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Efavirenz

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT)
MWexperimental 120 kDa
MWexpected 120 kDa
VPorod 178 nm3
log I(s) 1.10×100 1.10×10-1 1.10×10-2 1.10×10-3
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) small angle scattering data  s, nm-1
ln I(s)
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Guinier plot ln 1.10×100 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot 1.104 0 3 sRg
p(r)
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) pair distance distribution function Rg: 3.7 nm 0 Dmax: 11.3 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Efavirenz in 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH 7 were collected on the BL1.3W beam line at the Synchrotron Light Research Institute (SLRI) storage ring (Nakhon Ratchasima, Thailand) using a Rayonix SX165 detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.137 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.43 mg/ml was measured at 16°C. One 600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Efavirenz: https://pubchem.ncbi.nlm.nih.gov/compound/Efavirenz

Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) (p66)
Mol. type   Protein
Organism   Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)
Olig. state   Monomer
Mon. MW   66.6 kDa
 
UniProt   P04585 (588-1147)
Sequence   FASTA
 
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) (p51)
Mol. type   Protein
Organism   Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)
Olig. state   Monomer
Mon. MW   53.5 kDa
 
UniProt   P04585 (588-1027)
Sequence   FASTA