Synchrotron SAXS data from solutions of the D2 domain of E3 ubiquitin-protein ligase DTX3L in 30 mM HEPES, 350 mM NaCl, 5% (v/v) glycerol, 0.5 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 56.00 μl sample at 10 mg/ml was injected at a 0.06 ml/min flow rate onto a GE Superose 6 Increase 3.2/300 column at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION: Data are truncated at low-s (limited low-angle/Guinier region). Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN
s, nm-1
