Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family

Megalizzi V, Tanina A, Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R, Heliyon :e40494 (2024) DOI

SASDUA8 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - tetrameric peak

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)
Uncharacterized protein Rv2242
MWexperimental 392 kDa
MWexpected 332 kDa
VPorod 826 nm3
log I(s) 1.05×10-2 1.05×10-3 1.05×10-4 1.05×10-5
Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) Uncharacterized protein Rv2242 small angle scattering data  s, nm-1
ln I(s)
Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) Uncharacterized protein Rv2242 Guinier plot ln 1.06×10-2 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) Uncharacterized protein Rv2242 Kratky plot 1.104 0 √3 sRg
p(r)
Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) Uncharacterized protein Rv2242 pair distance distribution function Rg: 5.2 nm 0 Dmax: 15.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) Uncharacterized protein Rv2242 ALPHAFOLD model
Synchrotron SAXS data from solutions of MPB-MabR(Rv2242) fusion protein in 50 mM Tris pH 7.5, 150 mM NaCl were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 6.6 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) (MBP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Tetramer
Mon. MW   40.2 kDa
 
UniProt   P0AEX9 (27-384)
Sequence   FASTA
 
Uncharacterized protein Rv2242 (MabR)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Tetramer
Mon. MW   42.9 kDa
 
UniProt   P9WPH5 (19-409)
Sequence   FASTA