Yu M,
Gruzinov AY,
Ruan H,
Scheidt T,
Chowdhury A,
Giofrè S,
Mohammed ASA,
Caria J,
Sauter PF,
Svergun DI,
Lemke EA,
Proc Natl Acad Sci U S A
121(50):e2415220121
(2024)
Europe PMC
SASDUP9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea
Synchrotron SAXS data from solutions of Br-labelled IBB in phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 80.00 μl sample at 20 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 20°C. 2400 successive 0.250 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Br-labeled IBB in 2 M urea measured at 13428 eV. SAXS data measured at several different energies spanning the K-absorbtion edge of bromine are made available in the full entry zip archive.
s, nm-1
