SASDXF2 – Probable conserved Mce associated membrane protein (Mam1Adelta106) from M.tuberculosis

Probable conserved Mce associated membrane protein

MW^experimental	39	kDa
MW^expected	59	kDa
V^Porod	90	nm³

log I(s) 4.01×10^-2 4.01×10^-3 4.01×10^-4 4.01×10^-5

Probable conserved Mce associated membrane protein small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Probable conserved Mce associated membrane protein Guinier plot

ln 4.02×10^-2 R_g: 3.2 nm 0 (3.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

Probable conserved Mce associated membrane protein Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 3.2 nm 0 D_max: 90 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

2.2

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0
2.121

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Probable conserved Mce associated membrane protein ALPHAFOLD model

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of Probable conserved Mce associated membrane protein (Mam1Adelta106) from M.tuberculosis in 50mM Tris, 500mM NaCl, pH 8.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09464 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 35.00 μl sample at 8.8 mg/ml was injected at a 0.07 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 15°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Probable conserved Mce associated membrane protein (Mam1A delta 106)
Mol. type		Protein
Organism		Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state		Tetramer
Mon. MW		14.8 kDa

UniProt		O07419 (107-213)
Sequence		FASTA