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3 hits found for Azmi

SASDGN2 – F670E mutated dimeric bifunctional alcohol/aldehyde dehydrogenase

F670E Aldehyde-alcohol dehydrogenase experimental SAS data
SREFLEX model
Sample: F670E Aldehyde-alcohol dehydrogenase dimer, 192 kDa Escherichia coli protein
Buffer: 50 mM HEPES pH 7, 500 mM NaCl, 5% (v/v) glycerol, pH: 7
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Oct 4
Aldehyde-alcohol dehydrogenase forms a high-order spirosome architecture critical for its activity. Nat Commun 10(1):4527 (2019)
...Azmi L, Jang S, Jung T, Hebert H, Roe AJ, Byron O, Song JJ
RgGuinier 5.0 nm
Dmax 17.4 nm
VolumePorod 260 nm3

SASDC72 – Alcohol dehydrogenase from bifunctional alcohol/aldehyde dehydrogenase (P0A9Q8)

Aldehyde-alcohol dehydrogenase experimental SAS data
DAMMIF model
Sample: Aldehyde-alcohol dehydrogenase dimer, 97 kDa Escherichia coli O157:H7 protein
Buffer: 20 mM Tris 400 mM NaCl 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Nov 27
High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE. Acta Crystallogr F Struct Biol Commun 76(Pt 9):414-421 (2020)
Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M
RgGuinier 3.2 nm
Dmax 16.9 nm
VolumePorod 146 nm3

SASDCK3 – Aldehyde dehydrogenase

Aldehyde-alcohol dehydrogenase experimental SAS data
DAMMIF model
Sample: Aldehyde-alcohol dehydrogenase monomer, 48 kDa Escherichia coli O157:H7 protein
Buffer: 30 mM HEPES, 150 mM NaCl, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 30
High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE. Acta Crystallogr F Struct Biol Commun 76(Pt 9):414-421 (2020)
Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M
RgGuinier 2.7 nm
Dmax 11.1 nm
VolumePorod 74 nm3