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12 hits found for Cai

SASDDB3 – Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 204 nm3

SASDDC3 – Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrial experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 218 nm3

SASDDD3 – Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrialFrataxin, mitochondrial experimental SAS data
HADDOCK model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Frataxin, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 Apr 18
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 287 nm3

SASDCQ5 – SAXS on intact Doryteuthis squid lens 100% layer

S-crystallin, Q25367_DOROP experimental SAS data
DAMMIF model
Sample: S-crystallin, Q25367_DOROP dimer, 55 kDa Doryteuthis pealeii protein
Buffer: Tissue fluid, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 Jul 17
Eye patches: Protein assembly of index-gradient squid lenses. Science 357(6351):564-569 (2017)
Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM

SASDCR5 – SAXS on intact Doryteuthis squid lens 80% layer

S-crystallin, Q25367_DOROP experimental SAS data
DAMMIF model
Sample: S-crystallin, Q25367_DOROP dimer, 55 kDa Doryteuthis pealeii protein
Buffer: Tissue fluid, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 Jul 19
Eye patches: Protein assembly of index-gradient squid lenses. Science 357(6351):564-569 (2017)
Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM

SASDCS5 – SAXS on intact Doryteuthis squid lens 60% layer

S-crystallin, Q25367_DOROP experimental SAS data
DAMMIF model
Sample: S-crystallin, Q25367_DOROP dimer, 55 kDa Doryteuthis pealeii protein
Buffer: Tissue fluid, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 Jul 17
Eye patches: Protein assembly of index-gradient squid lenses. Science 357(6351):564-569 (2017)
Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM

SASDCT5 – SAXS on intact Doryteuthis squid lens 40% layer

S-crystallin, Q25367_DOROP experimental SAS data
DAMMIF model
Sample: S-crystallin, Q25367_DOROP dimer, 55 kDa Doryteuthis pealeii protein
Buffer: Tissue fluid, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 Jul 17
Eye patches: Protein assembly of index-gradient squid lenses. Science 357(6351):564-569 (2017)
Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM

SASDCU5 – SAXS dilution series on ground Doryteuthis squid lens 100% layer

S-crystallin, Q25367_DOROP experimental SAS data
S-crystallin, Q25367_DOROP Kratky plot
Sample: S-crystallin, Q25367_DOROP dimer, 55 kDa Doryteuthis pealeii protein
Buffer: PBS, phosphate buffered saline, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Nov 12
Eye patches: Protein assembly of index-gradient squid lenses. Science 357(6351):564-569 (2017)
Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM
RgGuinier 2.4 nm

SASDDV8 – Boiled chicken egg albumen

Ovalbumin experimental SAS data
Ovalbumin Kratky plot
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at Bruker Nonius FR591, University of Pennslyvania on 2013 Jun 27
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM

SASDDW8 – Chinese century egg albumen (pidan) made from quail egg

Ovalbumin (common quail) experimental SAS data
Ovalbumin (common quail) Kratky plot
Sample: Ovalbumin (common quail) monomer, 42 kDa Coturnix coturnix protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at Bruker Nonius FR591, University of Pennslyvania on 2013 Jun 27
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM

SASDDX8 – Raw chicken egg albumen

Ovalbumin experimental SAS data
Ovalbumin Kratky plot
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at Bruker Nonius FR591, University of Pennslyvania on 2013 Jun 27
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM

SASDDY8 – Chicken ovalbumin gel at high pH

Ovalbumin experimental SAS data
DAMMIF model
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: 0.16 M NaOH, pH: 13.2
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 Feb 21
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM