Small Angle Scattering Biological Data Bank SASBDB


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6 hits found for Cavini

SASDUV2 – MBP-fused Schistosoma mansoni Septin-10 C-terminal coiled-coil domain (MBP-SmSEPT10C)

Sample:	Septin-10 dimer, 110 kDa Schistosoma mansoni protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 May 17

Novel lipid-interaction motifs within the C-terminal domain of Septin10 from Schistosoma mansoni Biochimica et Biophysica Acta (BBA) - Biomembranes :184371 (2024)
Cavini I, Fontes M, Zeraik A, Lopes J, Araújo A

R_g^Guinier	5.7	nm
D_max	27.4	nm
Volume^Porod	175	nm³

SASDR39 – MBP-, SUMO-fused heterodimeric SEPT6-SEPT7 septin coiled coil (MBP-SEPT6c+SUMO-SEPT7c)

Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP)Septin-7 (C-terminal domain of SEPT7 fused to SUMO) experimental SAS data

Sample:	Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP) monomer, 55 kDa Homo sapiens protein Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC

R_g^Guinier	5.4	nm
D_max	21.5	nm
Volume^Porod	142	nm³

SASDR49 – MBP-, SUMO-fused heterodimeric SEPT8-SEPT7 septin coiled coil (MBP-SEPT8c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-8 (C-terminal domain of SEPT8 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-8 (C-terminal domain of SEPT8 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

R_g^Guinier	5.3	nm
D_max	19.9	nm
Volume^Porod	140	nm³

SASDR59 – MBP-, SUMO-fused heterodimeric SEPT10-SEPT7 septin coiled coil (MBP-SEPT10c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-10 (C-terminal domain of SEPT10 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-10 (C-terminal domain of SEPT10 fused to MBP) monomer, 56 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

R_g^Guinier	5.7	nm
D_max	21.7	nm
Volume^Porod	154	nm³

SASDR69 – MBP-, SUMO-fused heterodimeric SEPT11-SEPT7 septin coiled coil (MBP-SEPT11c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-11 (C-terminal domain of SEPT11 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-11 (C-terminal domain of SEPT11 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

R_g^Guinier	5.2	nm
D_max	20.9	nm
Volume^Porod	139	nm³

SASDR79 – MBP-, SUMO-fused heterodimeric SEPT14-SEPT7 septin coiled coil (MBP-SEPT14c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-14 (C-terminal domain of SEPT14 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-14 (C-terminal domain of SEPT14 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3