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47 hits found for Clement Blanchet

SASDFA3 – Human ATP-citrate synthase (ACLY) full length in HBS

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 19.0 nm
VolumePorod 765 nm3

SASDFB3 – Human ATP-citrate synthers (ACLY) full length in HBS + Citrate

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.2 nm
Dmax 19.0 nm
VolumePorod 787 nm3

SASDFC3 – Human ATP-citrate synthase (ACLY) full length in HBS + Citrate + Coenzyme-A

ATP-citrate synthase experimental SAS data
MULTIFOXS model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.9 nm
Dmax 17.0 nm
VolumePorod 775 nm3

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3

SASDAF4 – DmMfe2

Peroxisomal multifunctional enzyme type 2 experimental SAS data
Peroxisomal multifunctional enzyme type 2 Kratky plot
Sample: Peroxisomal multifunctional enzyme type 2 dimer, 128 kDa Drosophila melanogaster protein
Buffer: 20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 12
Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering. FEBS Lett 587(4):305-10 (2013)
...Blanchet CE, Hiltunen JK, Svergun DI, Glumoff T
RgGuinier 3.6 nm
Dmax 12.0 nm

SASDAG4 – HsMfe2

Peroxisomal multifunctional enzyme type 2 experimental SAS data
Peroxisomal multifunctional enzyme type 2 Kratky plot
Sample: Peroxisomal multifunctional enzyme type 2 dimer, 159 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 22
Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering. FEBS Lett 587(4):305-10 (2013)
...Blanchet CE, Hiltunen JK, Svergun DI, Glumoff T
RgGuinier 4.6 nm
Dmax 15.0 nm

SASDAJ4 – CRM1

Exportin-1 experimental SAS data
DAMMIF model
Sample: Exportin-1 monomer, 123 kDa Mus musculus protein
Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3
Structural determinants and mechanism of mammalian CRM1 allostery. Structure 21(8):1350-60 (2013)
...Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A
RgGuinier 3.8 nm
Dmax 11.0 nm
VolumePorod 190 nm3

SASDKJ4 – SARS-CoV-2 nsp7 and nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a - nsp7 , 18 kDa Severe acute respiratory … protein
Replicase polyprotein 1a - nsp8 , 44 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19
Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
...Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 92 nm3

SASDAK4 – CRM1 RanGTP

Exportin-1GTP-binding nuclear protein Ran experimental SAS data
DAMMIF model
Sample: Exportin-1 monomer, 123 kDa Mus musculus protein
GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3
Structural determinants and mechanism of mammalian CRM1 allostery. Structure 21(8):1350-60 (2013)
...Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A
RgGuinier 3.6 nm
Dmax 10.0 nm

SASDAL4 – CRM1 RanGTP Snu1

Exportin-1GTP-binding nuclear protein RanSnurportin-1 experimental SAS data
DAMMIF model
Sample: Exportin-1 monomer, 123 kDa Mus musculus protein
GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein
Snurportin-1 monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3
Structural determinants and mechanism of mammalian CRM1 allostery. Structure 21(8):1350-60 (2013)
...Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A
RgGuinier 4.1 nm
Dmax 14.0 nm

SASDAM4 – CRM1 Snu1

Exportin-1Snurportin-1 experimental SAS data
DAMMIF model
Sample: Exportin-1 monomer, 123 kDa Mus musculus protein
Snurportin-1 monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 10
Structural determinants and mechanism of mammalian CRM1 allostery. Structure 21(8):1350-60 (2013)
...Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A
RgGuinier 4.3 nm
Dmax 15.0 nm

SASDGV5 – The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)

Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain experimental SAS data
Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain monomer, 27 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.45 mM Mg2+-ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 8
Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering. Structure (2019)
...Blanchet CE, Schroer MA, Huse N, Pearson AR, Svergun DI, Tidow H
RgGuinier 2.1 nm
Dmax 6.8 nm
VolumePorod 50 nm3

SASDE36 – Human ATP-citrate synthase (ACLY) in HBS

ATP-citrate synthase experimental SAS data
MULTIFOXS model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.0 nm
Dmax 17.5 nm
VolumePorod 738 nm3

SASDE46 – Human ATP-citrate synthers (ACLY) in HBS + Citrate

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 17.5 nm
VolumePorod 747 nm3

SASDE56 – Human ATP-citrate synthase (ACLY) in HBS + Citrate + Coenzyme-A

ATP-citrate synthase experimental SAS data
SASREF CV model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 5
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.8 nm
Dmax 16.5 nm
VolumePorod 709 nm3

SASDE66 – C. limicola ATP-citrate lyase (ACL) in HBS

ATP-citrate lyase experimental SAS data
ATP-citrate lyase Kratky plot
Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 20.0 nm
VolumePorod 666 nm3

SASDE76 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate

ATP-citrate lyase experimental SAS data
ATP-citrate lyase Kratky plot
Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.0 nm
Dmax 20.0 nm
VolumePorod 672 nm3

SASDE86 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A

ATP-citrate lyase experimental SAS data
ATP-citrate lyase Kratky plot
Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.7 nm
Dmax 17.5 nm
VolumePorod 791 nm3

SASDF86 – Human Galectin-10 (Tyr69Glu mutant)

Galectin-10 Tyr69Glu experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Galectin-10 Tyr69Glu dimer, 33 kDa Homo sapiens protein
Buffer: 20 mM Hepes 150 NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Feb 4
Protein crystallization promotes type 2 immunity and is reversible by antibody treatment. Science 364(6442) (2019)
...Blanchetot C, Saunders M, Hammad H, Savvides SN, Lambrecht BN
RgGuinier 2.1 nm
Dmax 8.2 nm
VolumePorod 46 nm3

SASDPF7 – Ribonuclease A SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 16 nm3

SASDPG7 – Ribonuclease A SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 16 nm3

SASDPH7 – Urate Oxidase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 8.8 nm
VolumePorod 221 nm3

SASDPJ7 – Urate Oxidase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 214 nm3

SASDPK7 – Xylose isomerase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 14.1 nm
VolumePorod 228 nm3

SASDPL7 – Xylose isomerase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.3 nm
Dmax 14.1 nm
VolumePorod 233 nm3

SASDQL7 – Bicelles formed by DMPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 1
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDPM7 – Xylanase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.6 nm
Dmax 4.8 nm
VolumePorod 25 nm3

SASDQM7 – Bicelles formed by DMPC cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDPN7 – Xylanase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.6 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDQN7 – Bicelles formed by DMPC CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQP7 – Bicelles formed by DMPC DMPG and CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonateDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQQ7 – Bicelles formed by DMPC DMPG and cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQR7 – Bicelles formed by DMPC DMPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQS7 – Bicelles formed by POPC CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQT7 – Bicelles formed by POPC cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQU7 – Bicelles formed by POPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQV7 – Bicelles formed by POPC POPG and CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQW7 – Bicelles formed by POPC POPG and cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQX7 – Bicelles formed by POPC POPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDCQ8 – Bovine serum albumin (BSA)

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.8 nm

SASDCR8 – Glucose isomerase

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 100 mM HEPES, 1 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.7 nm

SASDCS8 – Cytochrome C

Cytochrome C experimental SAS data
Cytochrome C Kratky plot
Sample: Cytochrome C monomer, 12 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 1.3 nm

SASDQY8 – Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SANS data collected at SPB/SFX, European XFEL on 2021 May 15
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...
RgGuinier 2.7 nm

SASDQZ8 – Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Ferritin light chain experimental SAS data
Ferritin light chain Kratky plot
Sample: Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SANS data collected at SPB/SFX, European XFEL on 2021 May 14
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...
RgGuinier 5.8 nm

SASDQ29 – Thyroglobulin - reservoir sample storage with liquid jet delivery measured using XFEL

Thyroglobulin experimental SAS data
Thyroglobulin Kratky plot
Sample: Thyroglobulin monomer, 303 kDa Bos taurus protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SANS data collected at SPB/SFX, European XFEL on 2021 May 14
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...
RgGuinier 7.0 nm

SASDQ39 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) - reservoir sample storage with liquid jet delivery measured using XFEL

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data
Spike glycoprotein (ACE2 receptor binding domain) Kratky plot
Sample: Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SANS data collected at SPB/SFX, European XFEL on 2021 May 14
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...
RgGuinier 3.0 nm