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15 hits found for Garnett

SASDHW2 – The Legionella pneumophila type II secretion system substrate NttE

Uncharacterized protein experimental SAS data
GASBOR model
Sample: Uncharacterized protein dimer, 66 kDa Legionella pneumophila protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 18
Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System Frontiers in Molecular Biosciences 7 (2020)
...Garnett J
RgGuinier 2.9 nm
Dmax 9.4 nm
VolumePorod 114 nm3

SASDUG7 – Wild type Lcl C-terminal domain

HbP1 experimental SAS data
HbP1 Kratky plot
Sample: HbP1 trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 105 nm3

SASDUH7 – Lcl C-terminal domain R477A mutant - trimer

HbP1 (R477A) experimental SAS data
HbP1 (R477A) Kratky plot
Sample: HbP1 (R477A) trimer, 55 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.7 nm
Dmax 9.4 nm
VolumePorod 79 nm3

SASDUJ7 – Lcl C-terminal domain R477A mutant - monomer

HbP1 (R477A) experimental SAS data
HbP1 (R477A) Kratky plot
Sample: HbP1 (R477A) monomer, 18 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 1.9 nm
Dmax 6.4 nm
VolumePorod 33 nm3

SASDUK7 – Lcl C-terminal domain E503A mutant

HbP1 (E503A) experimental SAS data
HbP1 (E503A) Kratky plot
Sample: HbP1 (E503A) trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 106 nm3

SASDUL7 – Lcl C-terminal domain K504A mutant

HbP1 (K504A) experimental SAS data
HbP1 (K504A) Kratky plot
Sample: HbP1 (K504A) trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 106 nm3

SASDUM7 – Lcl C-terminal domain K515A mutant

HbP1 (K515A) experimental SAS data
HbP1 (K515A) Kratky plot
Sample: HbP1 (K515A) trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.8 nm
Dmax 9.8 nm
VolumePorod 105 nm3

SASDUN7 – Lcl C-terminal domain K520A mutant

HbP1 (K520A) experimental SAS data
HbP1 (K520A) Kratky plot
Sample: HbP1 (K520A) trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.8 nm
Dmax 9.8 nm
VolumePorod 100 nm3

SASDUP7 – Lcl C-terminal domain D521A mutant

HbP1 (D521A) experimental SAS data
HbP1 (D521A) Kratky plot
Sample: HbP1 (D521A) trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.8 nm
Dmax 9.8 nm
VolumePorod 108 nm3

SASDUQ7 – Lcl C-terminal domain K526A mutant

HbP1 (K526A) experimental SAS data
HbP1 (K526A) Kratky plot
Sample: HbP1 (K526A) trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
...Garnett JA
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 104 nm3

SASDLW2 – Accessory colonization factor SslE at pH 4.4

Accessory colonization factor experimental SAS data
DAMMIN model
Sample: Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein
Buffer: 20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 4.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 27
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
...Garnett J
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 248 nm3

SASDLV2 – Accessory colonization factor SslE at pH 7.4

Accessory colonization factor experimental SAS data
DAMMIN model
Sample: Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein
Buffer: 20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 27
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
...Garnett J
RgGuinier 4.0 nm
Dmax 14.1 nm
VolumePorod 244 nm3

SASDMU6 – The N1 domain of accessory colonization factor SslE

Accessory colonization factor experimental SAS data
DAMFILT model
Sample: Accessory colonization factor monomer, 17 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
...Garnett J
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3

SASDMV6 – The N2 domain of accessory colonization factor SslE

Accessory colonization factor experimental SAS data
DAMFILT model
Sample: Accessory colonization factor monomer, 23 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
...Garnett J
RgGuinier 2.2 nm
Dmax 7.7 nm
VolumePorod 42 nm3

SASDMW6 – The N3-M60 domain of accessory colonization factor SslE

Accessory colonization factor experimental SAS data
DAMFILT model
Sample: Accessory colonization factor monomer, 121 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
...Garnett J
RgGuinier 3.4 nm
Dmax 10.5 nm
VolumePorod 171 nm3