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10 hits found for Glover

SASDBR3 – Wild type RNF8 complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin

E3 ubiquitin-protein ligase RNF8Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-C experimental SAS data
MES-FOXS model
Sample: E3 ubiquitin-protein ligase RNF8 dimer, 35 kDa Homo sapiens protein
Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein
Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...Glover JN
RgGuinier 4.5 nm
Dmax 18.9 nm
VolumePorod 119 nm3

SASDBS3 – Wild type RNF8 complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin

E3 ubiquitin-protein ligase RNF8Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CUbiquitin-conjugating enzyme E2 variant 2 experimental SAS data
MES-FOXS model
Sample: E3 ubiquitin-protein ligase RNF8 dimer, 35 kDa Homo sapiens protein
Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein
Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Ubiquitin-conjugating enzyme E2 variant 2 dimer, 34 kDa Homo sapiens protein
Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...Glover JN
RgGuinier 5.4 nm
Dmax 23.8 nm
VolumePorod 214 nm3

SASDBT3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CE3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data
MES-FOXS model
Sample: Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein
Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein
Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...Glover JN
RgGuinier 4.3 nm
Dmax 18.9 nm
VolumePorod 111 nm3

SASDBU3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CUbiquitin-conjugating enzyme E2 variant 2E3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data
MES-FOXS model
Sample: Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein
Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Ubiquitin-conjugating enzyme E2 variant 2 dimer, 34 kDa Homo sapiens protein
E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein
Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...Glover JN
RgGuinier 5.2 nm
Dmax 23.8 nm
VolumePorod 192 nm3

SASDG76 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)Serine/threonine-protein phosphatase PP1-alpha catalytic subunit experimental SAS data
BILBOMD model
Sample: Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Glover JNM
RgGuinier 3.1 nm
Dmax 11.3 nm
VolumePorod 157 nm3

SASDG86 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)Serine/threonine-protein phosphatase PP1-alpha catalytic subunit experimental SAS data
BILBOMD model
Sample: Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Glover JNM
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 180 nm3

SASDG66 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitInhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 25
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Glover JNM
RgGuinier 3.0 nm
Dmax 10.9 nm
VolumePorod 116 nm3

SASDG56 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitInhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 25
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Glover JNM
RgGuinier 3.4 nm
Dmax 12.4 nm
VolumePorod 132 nm3

SASDG96 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitApoptosis-stimulating of p53 protein 2 experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Apoptosis-stimulating of p53 protein 2 monomer, 26 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Glover JNM
RgGuinier 2.9 nm
Dmax 10.2 nm
VolumePorod 144 nm3

SASDGA6 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitApoptosis-stimulating of p53 protein 2 experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Apoptosis-stimulating of p53 protein 2 monomer, 26 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Glover JNM
RgGuinier 3.3 nm
Dmax 11.7 nm
VolumePorod 172 nm3