Search

  
Advanced search  

12 hits found for Karen Manalastas-Cantos

SASDHZ9 – Condensin complex subunit 1, Ycs4

Condensin complex subunit 1 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 5.2 nm
Dmax 17.7 nm
VolumePorod 296 nm3

SASDJ22 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 336-418 (Brn1S)

Condensin complex subunit 1Condensin complex subunit 2, 336-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 336-418 monomer, 9 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 5.0 nm
Dmax 16.9 nm
VolumePorod 279 nm3

SASDJ32 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 225-418 (Brn1L)

Condensin complex subunit 1Condensin complex subunit 2, 225-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 4.9 nm
Dmax 15.9 nm
VolumePorod 309 nm3

SASDJ42 – Pentameric assembly of condensin complex subunits 1 and 2 (Ycs4-Brn1L-Brn1C) with subunits of the SMC hinge domain-containing protein (Smc4hd)

Condensin complex subunit 1Condensin complex subunit 2, 225-418Condensin complex subunit 2, 776-898SMC hinge domain-containing protein, 263-466SMC hinge domain-containing protein, 1367-1542 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 776-898 monomer, 14 kDa Chaetomium thermophilum protein
SMC hinge domain-containing protein, 263-466 monomer, 22 kDa Chaetomium thermophilum protein
SMC hinge domain-containing protein, 1367-1542 monomer, 20 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 5.1 nm
Dmax 17.9 nm
VolumePorod 355 nm3

SASDFC4 – Condensin complex subunit 3-like protein, Ycg1, extrapolated to infinite dilution

Condensin complex subunit 3-like protein experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.6 nm
Dmax 15.6 nm
VolumePorod 236 nm3

SASDFD4 – Condensin complex subunit 3-like protein,Ycg1, bound to condensin complex subunit 2, Brn1 (Ycg1-Brn1)

Condensin complex subunit 3-like proteinCondensin complex subunit 2 experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 2 monomer, 17 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.3 nm
Dmax 13.7 nm
VolumePorod 230 nm3

SASDFE4 – Condensin complex subunit 3-like protein, Ycg1, at 10mg/ml (tetramer)

Condensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 8.3 nm
Dmax 32.7 nm
VolumePorod 1080 nm3

SASDFF4 – Condensin complex subunit 3-like protein, Ycg1, at 5mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 6.8 nm
Dmax 29.8 nm
VolumePorod 720 nm3

SASDFG4 – Condensin complex subunit 3-like protein, Ycg1, at 2mg/ml (dimer)

Condensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 5.4 nm
Dmax 19.0 nm
VolumePorod 463 nm3

SASDFH4 – Condensin complex subunit 3-like protein, Ycg1, at 1mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 5.3 nm
Dmax 18.7 nm
VolumePorod 400 nm3

SASDFJ4 – Condensin complex subunit 3-like protein, Ycg1, at 0.5mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.7 nm
Dmax 16.1 nm
VolumePorod 301 nm3

SASDFK4 – Condensin complex subunit 3-like protein, Ycg1, at 0.25mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.6 nm
Dmax 15.0 nm
VolumePorod 280 nm3