Search

  
Advanced search  

88 hits found for Kraft

SASDDD7 – Apolipoprotein E2

Apolipoprotein E2 experimental SAS data
Apolipoprotein E2 Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
The molecular basis for Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
...Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L
RgGuinier 5.6 nm
Dmax 19.5 nm
VolumePorod 400 nm3

SASDGD7 – Apolipoprotein E3 bound to 330 µg/mL heparin

Apolipoprotein E3Heparin experimental SAS data
Apolipoprotein E3 Heparin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 7.3 nm
Dmax 25.9 nm

SASDGE7 – Apolipoprotein E3 bound to 110 µg/mL heparin

Apolipoprotein E3Heparin experimental SAS data
Apolipoprotein E3 Heparin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 7.5 nm
Dmax 25.8 nm

SASDGF7 – Apolipoprotein E3 bound to 37 µg/mL heparin

Apolipoprotein E3Heparin experimental SAS data
Apolipoprotein E3 Heparin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.9 nm
Dmax 23.9 nm

SASDGG7 – Apolipoprotein E3 bound to 12 µg/mL heparin

Apolipoprotein E3Heparin experimental SAS data
Apolipoprotein E3 Heparin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.5 nm
Dmax 22.0 nm

SASDGH7 – Apolipoprotein E3 bound to 4 µg/mL heparin

Apolipoprotein E3Heparin experimental SAS data
Apolipoprotein E3 Heparin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.3 nm
Dmax 20.8 nm

SASDGJ7 – Apolipoprotein E4 bound to 330 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 7.6 nm
Dmax 26.5 nm

SASDGK7 – Apolipoprotein E4 bound to 110 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 7.6 nm
Dmax 27.0 nm

SASDGL7 – Apolipoprotein E4 bound to 37 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 7.0 nm
Dmax 25.8 nm

SASDGM7 – Apolipoprotein E4 bound to 12 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.7 nm
Dmax 22.0 nm

SASDGN7 – Apolipoprotein E4 bound to 4 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.4 nm
Dmax 21.2 nm

SASDGP7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 330 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 20.8 nm

SASDGQ7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 110 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 21.2 nm

SASDGR7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 37 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 21.3 nm

SASDGS7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 12 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.5 nm

SASDGT7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 4 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.5 nm

SASDGU7 – Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 3.1 nm
Dmax 10.5 nm

SASDGV7 – Truncated Apolipoprotein E4 (1-191) bound to 37 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.6 nm
Dmax 8.2 nm

SASDGW7 – Truncated Apolipoprotein E4 (1-191) bound to 12 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.5 nm
Dmax 7.8 nm

SASDGX7 – Truncated Apolipoprotein E4 (1-191) bound to 4 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.4 nm
Dmax 8.0 nm

SASDGY7 – Apolipoprotein E4 (methylated) bound to 330 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.9 nm
Dmax 23.7 nm

SASDGZ7 – Apolipoprotein E4 (methylated) bound to 110 µg/mL heparin

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.8 nm
Dmax 23.7 nm

SASDG28 – Apolipoprotein E4 (methylated) bound to 37 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.4 nm
Dmax 22.2 nm

SASDG38 – Apolipoprotein E4 (methylated) bound to 12 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.3 nm
Dmax 20.8 nm

SASDG48 – Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.2 nm

SASDG58 – Apolipoprotein E2 bound to 330 µM Suramin

Apolipoprotein E2Suramin experimental SAS data
Apolipoprotein E2 Suramin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.2 nm
Dmax 17.8 nm

SASDG68 – Apolipoprotein E2 bound to 110 µM Suramin

Apolipoprotein E2Suramin experimental SAS data
Apolipoprotein E2 Suramin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.6 nm
Dmax 18.2 nm

SASDG78 – Apolipoprotein E2 bound to 37 µM Suramin

Apolipoprotein E2Suramin experimental SAS data
Apolipoprotein E2 Suramin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.9 nm
Dmax 19.8 nm

SASDG88 – Apolipoprotein E2 bound to 12 µM Suramin

Apolipoprotein E2Suramin experimental SAS data
Apolipoprotein E2 Suramin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.3 nm

SASDD98 – Apolipoprotein E3

Apolipoprotein E3 experimental SAS data
Apolipoprotein E3 Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
The molecular basis for Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
...Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L
RgGuinier 5.7 nm
Dmax 19.5 nm
VolumePorod 410 nm3

SASDG98 – Apolipoprotein E2 bound to 4 µM Suramin

Apolipoprotein E2Suramin experimental SAS data
Apolipoprotein E2 Suramin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.5 nm

SASDDA8 – Apolipoprotein E4

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
The molecular basis for Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
...Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L
RgGuinier 5.8 nm
Dmax 19.6 nm
VolumePorod 430 nm3

SASDGA8 – Apolipoprotein E3 bound to 330 µM Suramin

Apolipoprotein E3Suramin experimental SAS data
Apolipoprotein E3 Suramin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.2 nm
Dmax 18.6 nm

SASDGB8 – Apolipoprotein E3 bound to 110 µM Suramin

Apolipoprotein E3Suramin experimental SAS data
Apolipoprotein E3 Suramin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.6 nm
Dmax 19.1 nm

SASDGC8 – Apolipoprotein E3 bound to 37 µM Suramin

Apolipoprotein E3Suramin experimental SAS data
Apolipoprotein E3 Suramin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.0 nm
Dmax 20.3 nm

SASDGD8 – Apolipoprotein E3 bound to 12 µM Suramin

Apolipoprotein E3Suramin experimental SAS data
Apolipoprotein E3 Suramin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.0 nm

SASDGE8 – Apolipoprotein E3 bound to 4 µM Suramin

Apolipoprotein E3Suramin experimental SAS data
Apolipoprotein E3 Suramin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 20.1 nm

SASDGF8 – Apolipoprotein E4 bound to 330 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.4 nm
Dmax 19.6 nm

SASDGG8 – Apolipoprotein E4 bound to 110 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.5 nm
Dmax 19.5 nm

SASDGH8 – Apolipoprotein E4 bound to 37 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.0 nm
Dmax 20.1 nm

SASDGJ8 – Apolipoprotein E4 bound to 12 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.1 nm

SASDGK8 – Apolipoprotein E4 bound to 4 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 20.4 nm

SASDGL8 – Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.0 nm
Dmax 22.4 nm

SASDGM8 – Apolipoprotein E4 (K143A K146A) mutant bound to 110 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.5 nm
Dmax 18.9 nm

SASDGN8 – Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.9 nm
Dmax 19.9 nm

SASDGP8 – Apolipoprotein E4 (K143A K146A) mutant bound to12 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.0 nm
Dmax 20.2 nm

SASDGQ8 – Apolipoprotein E4 (K143A K146A) mutant bound to 4 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.4 nm

SASDGR8 – Apolipoprotein E4 (methylated) bound to 330 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.0 nm
Dmax 20.6 nm

SASDGS8 – Apolipoprotein E4 (methylated) bound to 110 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.7 nm
Dmax 18.1 nm

SASDGT8 – Apolipoprotein E4 (methylated) bound to 37 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.8 nm
Dmax 19.6 nm

SASDGU8 – Apolipoprotein E4 (methylated) bound to 12 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.0 nm
Dmax 19.4 nm

SASDFV8 – Apolipoprotein E2 (ApoE2) bound to 4 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 20.7 nm
VolumePorod 504 nm3

SASDGV8 – Apolipoprotein E4 (methylated) bound to 4 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.0 nm
Dmax 19.7 nm

SASDFW8 – Apolipoprotein E2 (ApoE2) bound to 12 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.4 nm
Dmax 21.7 nm
VolumePorod 531 nm3

SASDGW8 – Truncated Apolipoprotein E4 (1-191) bound to 330 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 3.1 nm
Dmax 11.1 nm

SASDSW8 – Apo form of vaccinia virus polymerase holoenzyme E9-A20-D4

DNA polymeraseDNA polymerase processivity factor component OPG148Uracil-DNA glycosylase experimental SAS data
DNA polymerase DNA polymerase processivity factor component OPG148 Uracil-DNA glycosylase Kratky plot
Sample: DNA polymerase monomer, 117 kDa Vaccinia virus (strain … protein
DNA polymerase processivity factor component OPG148 monomer, 49 kDa Vaccinia virus (strain … protein
Uracil-DNA glycosylase monomer, 27 kDa Vaccinia virus (strain … protein
Buffer: 200 mM NaCl, 20 mM Tris-HCl and 1 mM TCEP, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 25
Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus. PLoS Pathog 20(5):e1011652 (2024)
...Kraft C, Grimm C, Böttcher B, Fischer U, Tarbouriech N, Iseni F
RgGuinier 5.0 nm
Dmax 18.0 nm
VolumePorod 350 nm3

SASDFX8 – Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.5 nm
Dmax 23.4 nm
VolumePorod 584 nm3

SASDGX8 – Truncated Apolipoprotein E4 (1-191) bound to 110 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.8 nm
Dmax 9.5 nm

SASDFY8 – Apolipoprotein E2 (ApoE2) bound to 110 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 7.1 nm
Dmax 24.0 nm
VolumePorod 666 nm3

SASDGY8 – Truncated Apolipoprotein E4 (1-191) bound to 37 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.6 nm
Dmax 8.0 nm

SASDFZ8 – Apolipoprotein E2 (ApoE2) bound to 330 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 7.2 nm
Dmax 24.5 nm
VolumePorod 696 nm3

SASDGZ8 – Truncated Apolipoprotein E4 (1-191) bound to 12 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.5 nm
Dmax 8.2 nm

SASDG29 – Truncated Apolipoprotein E4 (1-191) bound to 4 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.4 nm
Dmax 7.8 nm

SASDG39 – Apolipoprotein E2

Apolipoprotein E2 experimental SAS data
Apolipoprotein E2 Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 20.5 nm

SASDG49 – Apolipoprotein E3

Apolipoprotein E3 experimental SAS data
Apolipoprotein E3 Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.6 nm

SASDG59 – Apolipoprotein E4

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 20.8 nm

SASDG69 – Apolipoprotein E4 (K143A K146A) mutant

Apolipoprotein E4 (K143A K146A) mutant experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 20.7 nm

SASDG79 – Apolipoprotein E4 (methylated)

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 20.3 nm

SASDG89 – Truncated Apolipoprotein E4 (1-191)

Apolipoprotein E4 (1-191) experimental SAS data
Apolipoprotein E4 (1-191) Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.4 nm
Dmax 7.7 nm

SASDG99 – Apolipoprotein E2 (SEC-SAXS)

Apolipoprotein E2 experimental SAS data
Apolipoprotein E2 Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.8 nm
Dmax 20.0 nm

SASDGA9 – Apolipoprotein E2 bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 21.0 nm

SASDGB9 – Apolipoprotein E2 bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E2Suramin experimental SAS data
Apolipoprotein E2 Suramin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.1 nm
Dmax 18.3 nm

SASDGC9 – Apolipoprotein E3 (SEC-SAXS)

Apolipoprotein E3 experimental SAS data
Apolipoprotein E3 Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.8 nm
Dmax 20.0 nm

SASDGD9 – Apolipoprotein E3 bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E3Heparin experimental SAS data
Apolipoprotein E3 Heparin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.1 nm
Dmax 21.3 nm

SASDGE9 – Apolipoprotein E3 bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E3Suramin experimental SAS data
Apolipoprotein E3 Suramin Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.1 nm
Dmax 19.3 nm

SASDGF9 – Apolipoprotein E4 (SEC-SAXS)

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.9 nm
Dmax 20.0 nm

SASDGG9 – Apolipoprotein E4 bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.2 nm
Dmax 21.6 nm

SASDGH9 – Apolipoprotein E4 bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.6 nm
Dmax 18.7 nm

SASDGJ9 – Apolipoprotein E4 (methylated) - (SEC-SAXS)

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.9 nm
Dmax 20.0 nm

SASDGK9 – Apolipoprotein E4 (methylated) bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 6.4 nm
Dmax 21.7 nm

SASDGL9 – Apolipoprotein E4 (methylated) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.3 nm
Dmax 18.5 nm

SASDGM9 – Mutant Apolipoprotein E4 (K143A K146A) - (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutant experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.8 nm
Dmax 20.2 nm

SASDGN9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.7 nm
Dmax 19.8 nm

SASDGP9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 5.4 nm
Dmax 18.3 nm

SASDGQ9 – Truncated Apolipoprotein E4 (1-191) - (SEC-SAXS)

Apolipoprotein E4 (1-191) experimental SAS data
Apolipoprotein E4 (1-191) Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDGR9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 4.2 nm
Dmax 16.8 nm

SASDGS9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.4 nm
Dmax 8.3 nm

SASDGT9 – Truncated Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
...Kraft
RgGuinier 2.7 nm
Dmax 8.3 nm