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32 hits found for Martha Brennich

SASDEF2 – Mitochondrial import inner membrane translocase complex TIM9·10

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CORAL model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDEG2 – Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial GTP/GDP carrier protein 1 experimental SAS data
DAMMIF model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial GTP/GDP carrier protein 1 monomer, 33 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, imidiazole, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P
RgGuinier 4.5 nm
Dmax 16.0 nm
VolumePorod 272 nm3

SASDD93 – ATP-dependent Clp protease ATP-binding subunit ClpC1

ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
OTHER model
Sample: ATP-dependent Clp protease ATP-binding subunit ClpC1 , 95 kDa Mycobacterium tuberculosis protein
Buffer: Hepes 50 mM pH 7.5, KCl 100 mM, glycerol 10%, MgCl2 4 mM and ATP 1 mM, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 18
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis. J Biol Chem 293(22):8379-8393 (2018)
...Brennich M, Kazmaier U, Lelievre J, Ballell L, Goldberg A, Schanda P, Fraga H
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 2156 nm3

SASDDA3 – ATP-dependent Clp protease ATP-binding subunit ClpC1, second state

ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
ATP-dependent Clp protease ATP-binding subunit ClpC1 Kratky plot
Sample: ATP-dependent Clp protease ATP-binding subunit ClpC1 , 95 kDa Mycobacterium tuberculosis protein
Buffer: Hepes 50 mM pH 7.5, KCl 100 mM, glycerol 10%, MgCl2 4 mM and ATP 1 mM, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 18
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis. J Biol Chem 293(22):8379-8393 (2018)
...Brennich M, Kazmaier U, Lelievre J, Ballell L, Goldberg A, Schanda P, Fraga H
RgGuinier 7.9 nm
Dmax 25.1 nm
VolumePorod 2416 nm3

SASDEY3 – Tryparedoxin, reduced state

Tryparedoxin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.8 nm
VolumePorod 27 nm3

SASDEZ3 – Tryparedoxin, oxidized state

Tryparedoxin experimental SAS data
GASBOR model
Sample: Tryparedoxin monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.5 nm
VolumePorod 27 nm3

SASDE24 – Tryparedoxin, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin experimental SAS data
SASREF MX model
Sample: Tryparedoxin , 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 45 nm3

SASDE34 – Tryparedoxin W39A, reduced state

Tryparedoxin W39A experimental SAS data
OTHER model
Sample: Tryparedoxin W39A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 23 nm3

SASDE44 – Tryparedoxin W39A, oxidized state

Tryparedoxin W39A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W39A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 23 nm3

SASDE54 – Tryparedoxin W39A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin W39A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W39A , 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.8 nm
VolumePorod 26 nm3

SASDE64 – Tryparedoxin W70A, reduced state

Tryparedoxin W70A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W70A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 4.9 nm
VolumePorod 26 nm3

SASDE74 – Tryparedoxin W70A, oxidized state

Tryparedoxin W70A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W70A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.7 nm
Dmax 7.7 nm
VolumePorod 31 nm3

SASDE84 – Tryparedoxin W70A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin W70A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W70A , 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 7.2 nm
VolumePorod 42 nm3

SASDE94 – Tryparedoxin I109A, reduced state

Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 25 nm3

SASDEA4 – Tryparedoxin I109A, oxidized state

Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.7 nm
Dmax 4.3 nm
VolumePorod 27 nm3

SASDEB4 – Tryparedoxin I109A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A , 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 42 nm3

SASDEF4 – Tryparedoxin K102E, reduced state

Tryparedoxin K102E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin K102E monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 29 nm3

SASDEG4 – Tryparedoxin K102E, oxidized state

Tryparedoxin K102E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin K102E monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 4.3 nm
VolumePorod 26 nm3

SASDEH4 – Tryparedoxin K102E, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin K102E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin K102E , 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
...Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.8 nm
Dmax 6.2 nm
VolumePorod 37 nm3

SASDBT4 – Bovine serum albumin monomer (measured by SEC-SAXS)

Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Bovine Serum Albumin measured by SEC-SAXS
Martha Brennich
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 118 nm3

SASDCW5 – Dimeric Sortilin at pH 7.4

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
DAMMIF model
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 275 nm3

SASDCX5 – Monomeric Sortilin at pH 5.5

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E experimental SAS data
DAMMIF model
Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, 76 kDa Mus musculus protein
Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.4 nm
Dmax 10.0 nm
VolumePorod 217 nm3

SASDCY5 – Dimeric Sortilin at pH 5.5

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
CORAL model
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 336 nm3

SASDCZ5 – Monomeric Sortilin at pH 7.4

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E experimental SAS data
CORAL model
Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, 76 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 217 nm3

SASDEC7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4 without Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
...Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 134 nm3

SASDED7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4, 2mM Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
...Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 134 nm3

SASDCE7 – Monomeric Sortilin at pH 7.4 in the presence of neurotensin

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
Sortilin, also: Neurotensin-receptor 3 Kratky plot
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.3 nm
Dmax 11.7 nm
VolumePorod 192 nm3

SASDEE7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5, 0.5 mM Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM Hepes, pH 6.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
...Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 132 nm3

SASDCF7 – Dimeric Sortilin at pH 7.4 in the presence of neurotensin

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
Sortilin, also: Neurotensin-receptor 3 Kratky plot
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.7 nm
Dmax 13.5 nm
VolumePorod 253 nm3

SASDEF7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5 without Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM HEPES pH 6.5 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
...Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 140 nm3

SASDEG7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5 without Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein
Buffer: 10 mM Hepes, pH 4.5, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
...Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.7 nm
Dmax 10.0 nm
VolumePorod 285 nm3

SASDEH7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5, 0.5 mM Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein
Buffer: 10 mM Hepes, pH 4.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 4.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
...Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.7 nm
Dmax 10.0 nm
VolumePorod 280 nm3