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16 hits found for Metcalfe

SASDGG2 – Interleukin 11 receptor subunit alpha

Interleukin-11 receptor subunit alpha experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 41 nm3

SASDGH2 – Interleukin 11, N-terminally truncated

Interleukin 11 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Interleukin 11 monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.7 nm
Dmax 5.4 nm
VolumePorod 22 nm3

SASDGJ2 – Interleukin 11, full length

Interleukin 11 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Interleukin 11 monomer, 19 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.9 nm
Dmax 6.1 nm
VolumePorod 28 nm3

SASDGK2 – Interleukin 11/Interleukin 11 receptor alpha complex

Interleukin-11 receptor subunit alphaInterleukin 11 experimental SAS data
OTHER model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Interleukin 11 monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
The structure of the extracellular domains of human interleukin 11 α-receptor reveals mechanisms of cytokine engagement Journal of Biological Chemistry :jbc.RA119.012351 (2020)
Metcalfe R, Aizel K, Zlatic C, Nguyen P, Morton C, Lio D, Cheng H, Dobson R, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 3.3 nm
Dmax 10.2 nm
VolumePorod 84 nm3

SASDMC4 – Plasmodium falciparum tyrosyl-tRNA synthetase

Tyrosine--tRNA ligase experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
...Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S...
RgGuinier 3.7 nm
Dmax 12.1 nm
VolumePorod 126 nm3

SASDMD4 – Plasmodium falciparum tyrosyl-tRNA synthetase, S234C mutant

Tyrosine--tRNA ligase (S234C) experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase (S234C) dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
...Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S...
RgGuinier 3.7 nm
Dmax 12.0 nm
VolumePorod 124 nm3

SASDME4 – Human tyrosyl tRNA synthetase

Tyrosine--tRNA ligase, cytoplasmic experimental SAS data
OTHER model
Sample: Tyrosine--tRNA ligase, cytoplasmic dimer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4
Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
...Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S...
RgGuinier 3.6 nm
Dmax 11.5 nm
VolumePorod 118 nm3

SASDES6 – The 11S subunit of the Plasmodium falciparum proteasome, PA28

Proteasome activator PA28 experimental SAS data
CORAL model
Sample: Proteasome activator PA28 heptamer, 232 kDa Plasmodium falciparum protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 2
The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis. Nat Microbiol 4(11):1990-2000 (2019)
...Metcalfe RD, Hanssen E, Yang T, Gillett DL, Leis AP, Morton CJ, Kuiper MJ, Parker MW, Spillman NJ, Wong W, Tsu C, Dick LR, Griffin MDW, Tilley L
RgGuinier 4.3 nm
Dmax 12.9 nm
VolumePorod 484 nm3

SASDLG7 – LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94)

Relaxin receptor 1 experimental SAS data
LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94) Rg histogram
Sample: Relaxin receptor 1 monomer, 8 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6
Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2. J Mol Biol 433(21):167217 (2021)
...Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR
RgGuinier 2.1 nm
Dmax 10.0 nm
VolumePorod 19 nm3

SASDLH7 – LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105)

Relaxin receptor 2 experimental SAS data
LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105) Rg histogram
Sample: Relaxin receptor 2 monomer, 7 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6
Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2. J Mol Biol 433(21):167217 (2021)
...Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR
RgGuinier 1.7 nm
Dmax 7.1 nm
VolumePorod 12 nm3

SASDLM3 – Interleukin-11 signalling complex, truncated gp130

Interleukin-6 receptor subunit betaInterleukin-11Interleukin-11 receptor subunit alpha experimental SAS data
OTHER model
Sample: Interleukin-6 receptor subunit beta dimer, 69 kDa Homo sapiens protein
Interleukin-11 dimer, 36 kDa Homo sapiens protein
Interleukin-11 receptor subunit alpha dimer, 64 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 5.3 nm
Dmax 17.6 nm
VolumePorod 405 nm3

SASDLN3 – Interleukin 11 signalling complex, full-length extracellular gp130

Interleukin-11Interleukin-11 receptor subunit alphaInterleukin-6 receptor subunit beta experimental SAS data
OTHER model
Sample: Interleukin-11 dimer, 36 kDa Homo sapiens protein
Interleukin-11 receptor subunit alpha dimer, 64 kDa Homo sapiens protein
Interleukin-6 receptor subunit beta dimer, 134 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 28
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 6.2 nm
Dmax 20.7 nm
VolumePorod 710 nm3

SASDLP3 – Interleukin 11 complex with IL-11Rα and gp130 D2-D3

Interleukin-11 receptor subunit alphaInterleukin 11Interleukin-6 receptor subunit beta experimental SAS data
OTHER model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Interleukin 11 monomer, 18 kDa Homo sapiens protein
Interleukin-6 receptor subunit beta monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 3.6 nm
Dmax 12.9 nm
VolumePorod 127 nm3

SASDLS3 – Interleukin 11 Mutein, complex with gp130 (domains 1-3) and IL-11Rα

Interleukin-11 receptor subunit alphaInterleukin 11 MuteinInterleukin-6 receptor subunit beta experimental SAS data
DAMMIN model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Interleukin 11 Mutein monomer, 18 kDa Homo sapiens protein
Interleukin-6 receptor subunit beta monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 4.4 nm
Dmax 15.6 nm
VolumePorod 160 nm3

SASDLR3 – Interleukin 11 Mutein

Interleukin 11 Mutein experimental SAS data
OTHER model
Sample: Interleukin 11 Mutein monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.8 nm
Dmax 5.4 nm
VolumePorod 25 nm3

SASDLQ3 – Interleukin 11, W168A mutant

Interleukin-11 (W168A) experimental SAS data
OTHER model
Sample: Interleukin-11 (W168A) monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 28
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.7 nm
Dmax 5.2 nm
VolumePorod 22 nm3