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10 hits found for Miele

SASDJ46 – Fep1-rec-GATA

GATA-type iron responsive transcription factor Fep1 reconstituted24-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 reconstituted 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 13.1 nm
VolumePorod 73 nm3

SASDJ56 – Fep1-GATA

GATA-type iron responsive transcription factor Fep124-mer double strand DNA from the GATA promoter experimental SAS data
GATA-type iron responsive transcription factor Fep1 24-mer double strand DNA from the GATA promoter Kratky plot
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 85 nm3

SASDJ66 – GATA double strand DNA

24-mer double strand DNA from the GATA promoter experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 10 nm3

SASDJ76 – Fep1 wild type with [2Fe2S] cluster reconstituted

GATA-type iron responsive transcription factor Fep1 reconstituted experimental SAS data
Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.8 nm
Dmax 16.5 nm
VolumePorod 53 nm3

SASDJ86 – Fep1 wild type

GATA-type iron responsive transcription factor Fep1 experimental SAS data
Fep1 wild type Rg histogram
Sample: GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 5
Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC
RgGuinier 3.5 nm
Dmax 11.8 nm
VolumePorod 41 nm3

SASDL69 – Ectodomain of human syndecan-1

Syndecan-1 experimental SAS data
Ectodomain of human syndecan-1 Rg histogram
Sample: Syndecan-1 monomer, 27 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Sep 26
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
...Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 5.3 nm

SASDL79 – Ectodomain of human syndecan-2

Syndecan-2 experimental SAS data
Ectodomain of human syndecan-2 Rg histogram
Sample: Syndecan-2 monomer, 17 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 31
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
...Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 4.3 nm

SASDL89 – Ectodomain of human syndecan-3

Syndecan-3 experimental SAS data
Ectodomain of human syndecan-3 Rg histogram
Sample: Syndecan-3 monomer, 39 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 May 29
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
...Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 6.5 nm

SASDL99 – Ectodomain of human syndecan-4 isoform-2 Monomer

Syndecan-4 experimental SAS data
Ectodomain of human syndecan-4 isoform-2 Monomer Rg histogram
Sample: Syndecan-4 monomer, 18 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Jul 4
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
...Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 4.2 nm

SASDLA9 – Ectodomain of human syndecan-4 isoform-2 Dimer

Syndecan-4 experimental SAS data
Syndecan-4 Kratky plot
Sample: Syndecan-4 dimer, 36 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Jul 4
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
...Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 5.9 nm