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3 hits found for Ramsay

SASDPK4 – His-tagged RdfS Excisionase monomeric in solution

DNA-binding protein experimental SAS data
His-tagged RdfS Excisionase monomeric in solution Rg histogram
Sample: DNA-binding protein monomer, 13 kDa Mesorhizobium loti protein
Buffer: 150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 19
Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element Acta Crystallographica Section D Structural Biology 78(10) (2022)
...Ramsay J, Bond C
RgGuinier 2.2 nm
Dmax 9.2 nm
VolumePorod 23 nm3

SASDW97 – Recombination directionality factor (6H-RdfS) from M.japonicum bound to 40mer DNA

Recombination directionality factor RdfSattP_8 40-mer DNA experimental SAS data
OTHER model
Sample: Recombination directionality factor RdfS tetramer, 52 kDa Mesorhizobium japonicum R7A protein
attP_8 40-mer DNA dimer, 25 kDa DNA
Buffer: 150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 19
Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix–turn–helix protein RdfS Nucleic Acids Research 53(6) (2025)
...Ramsay J
RgGuinier 3.8 nm
Dmax 13.8 nm
VolumePorod 134 nm3

SASDNM8 – Quorum sensing master protein (QseM)

DUF2285 domain-containing protein experimental SAS data
DUF2285 domain-containing protein Kratky plot
Sample: DUF2285 domain-containing protein monomer, 11 kDa Mesorhizobium loti protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 1
DUF2285 is a novel helix-turn-helix domain variant that orchestrates both activation and antiactivation of conjugative element transfer in proteobacteria. Nucleic Acids Res (2023)
...Ramsay JP, Ronson CW
RgGuinier 1.5 nm
Dmax 5.7 nm
VolumePorod 15 nm3