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18 hits found for Raphael Dos Santos Morais

SASDFZ9 – RuvB-like 1 and 2, sperm-associated antigen 1 complex (His-RuvBL1/RuvBL2/SPAG1)

RuvB-like 1RuvB-like 2Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: RuvB-like 1 hexamer, 311 kDa Homo sapiens protein
RuvB-like 2 hexamer, 311 kDa Homo sapiens protein
Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 6.8 nm
Dmax 34.8 nm
VolumePorod 1800 nm3

SASDG22 – RuvB-like 1 and 2 dodecamer (His-RuvBL1/RuvBL2)

RuvB-like 1RuvB-like 2 experimental SAS data
DAMMIF model
Sample: RuvB-like 1 hexamer, 311 kDa Homo sapiens protein
RuvB-like 2 hexamer, 311 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 3
R2SP
Raphael Dos Santos Morais
RgGuinier 6.3 nm
Dmax 23.4 nm
VolumePorod 1550 nm3

SASDG32 – Sperm-associated antigen 1, PIH1 domain-containing protein 2 heterodimer complex (SPAG1/PIH1D2)

Sperm-associated antigen 1PIH1 domain-containing protein 2 experimental SAS data
DAMMIF model
Sample: Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
PIH1 domain-containing protein 2 monomer, 36 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 7.0 nm
Dmax 30.9 nm
VolumePorod 664 nm3

SASDG42 – Truncated sperm-associated antigen 1 (622-926), PIH1 domain-containing protein 2 heterodimer complex (SPAG1(622-926)/PIH1D2)

PIH1 domain-containing protein 2Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: PIH1 domain-containing protein 2 monomer, 36 kDa Homo sapiens protein
Sperm-associated antigen 1 monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 5.0 nm
Dmax 19.3 nm
VolumePorod 123 nm3

SASDGM2 – R16-24 del44-54 human dystrophin fragment

Human dystrophin central domain R16-24 del44-54 fragment experimental SAS data
Human dystrophin central domain R16-24 del44-54 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del44-54 fragment monomer, 57 kDa Homo sapiens protein
Buffer: 20 mM Na-phosphate, 300 mM NaCl, 1 mM EDTA, 2% glycerol,, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 8
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 6.4 nm
Dmax 25.0 nm
VolumePorod 159 nm3

SASDFY4 – R4-15 human dystrophin fragment

Human dystrophin central domain R4-15 fragment experimental SAS data
Human dystrophin central domain R4-15 fragment Kratky plot
Sample: Human dystrophin central domain R4-15 fragment monomer, 150 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 May 26
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 9.7 nm
Dmax 47.5 nm

SASDFZ4 – R16-24 human dystrophin fragment

Human dystrophin central domain R16-24 fragment experimental SAS data
Human dystrophin central domain R16-24 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 fragment monomer, 127 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Dec 17
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 9.3 nm
Dmax 34.0 nm

SASDF25 – R16-24 del45-55 human dystrophin fragment

Human dystrophin central domain R16-24 del45-55 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-55 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-55 fragment monomer, 58 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Sep 28
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 4.7 nm
Dmax 18.3 nm

SASDF35 – R16-24 del45-51 human dystrophin fragment

Human dystrophin central domain R16-24 del45-51 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-51 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-51 fragment monomer, 85 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Jul 13
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 7.0 nm
Dmax 26.2 nm

SASDFY9 – Sperm-associated antigen 1 (SPAG1)

Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 7.3 nm
Dmax 34.0 nm
VolumePorod 374 nm3

SASDFW4 – Conformation of R8-15 human dystrophin fragment

Human dystrophin central domain R8-15 fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Human dystrophin central domain R8-15 fragment monomer, 100 kDa protein
Buffer: NaP 10 mM, NaCl 500 mM, EDTA 1 mM, Glycerol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Sep 23
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
...Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 10.1 nm
Dmax 36.0 nm

SASDFF5 – Protein DPCD

Protein DPCD experimental SAS data
DAMMIF model
Sample: Protein DPCD monomer, 24 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. J Mol Biol :167760 (2022)
Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B,...
RgGuinier 2.2 nm
Dmax 12.3 nm
VolumePorod 39 nm3

SASDFX4 – Conformation of R11-19 human dystrophin fragment

Human dystrophin central domain R11-19 fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Human dystrophin central domain R11-19 fragment monomer, 117 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycerol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 May 11
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
...Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 8.8 nm
Dmax 37.5 nm
VolumePorod 513 nm3

SASDFH5 – DPCD His-RuvBl1/RuvBl2 hexamer complex

Protein DPCDRuvB-like 1RuvB-like 2 experimental SAS data
GASBOR model
Sample: Protein DPCD trimer, 71 kDa Homo sapiens protein
RuvB-like 1 trimer, 155 kDa Homo sapiens protein
RuvB-like 2 trimer, 156 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. J Mol Biol :167760 (2022)
Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B,...
RgGuinier 5.4 nm
Dmax 15.9 nm
VolumePorod 856 nm3

SASDFT4 – Conformation of the R11-15 human dystrophin fragment (SANS)

Dystrophin (R11-15 human dystrophin fragment) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Dystrophin (R11-15 human dystrophin fragment) monomer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% v/v D2O, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 1
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
...Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 6.2 nm
Dmax 27.4 nm
VolumePorod 146 nm3

SASDFG5 – His-RuvBl1/RuvBl2 dodecamer

RuvB-like 1RuvB-like 2 experimental SAS data
DAMMIF model
Sample: RuvB-like 1 hexamer, 311 kDa Homo sapiens protein
RuvB-like 2 hexamer, 311 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. J Mol Biol :167760 (2022)
Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B,...
RgGuinier 6.2 nm
Dmax 20.0 nm
VolumePorod 1490 nm3

SASDFU4 – Conformation of R11-15 human dystrophin fragment in interaction with zwitterionic phospholipid bicelles (SANS)

Dystrophin (R11-15 human dystrophin fragment) experimental SAS data
DAMMIF model
Sample: Dystrophin (R11-15 human dystrophin fragment) monomer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% v/v D2O, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 1
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
...Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 6.2 nm
Dmax 28.1 nm
VolumePorod 144 nm3

SASDFV4 – Conformation of R11-15 human dystrophin fragment in interaction with anionic phospholipid bicelles (SANS)

Dystrophin (R11-15 human dystrophin fragment) experimental SAS data
DAMMIF model
Sample: Dystrophin (R11-15 human dystrophin fragment) monomer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% v/v D2O, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 1
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
...Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 8.1 nm
Dmax 29.6 nm
VolumePorod 231 nm3