Small Angle Scattering Biological Data Bank SASBDB


Advanced search

7 hits found for Rasmussen

SASDEF3 – Paenibacillus xanthan lyase (PXL) at 4 °C

Paenibacillus xanthan lyase experimental SAS data

Sample:	Paenibacillus xanthan lyase monomer, 113 kDa Paenibacillus sp-62047 protein
Buffer:	20 mM Tris,, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Dec 15

Structure and Dynamics of a Promiscuous Xanthan Lyase from Paenibacillus nanensis and the Design of Variants with Increased Stability and Activity. Cell Chem Biol 26(2):191-202.e6 (2019)
...Rasmussen KK, Agarwal S, Sainathan RK, Monrad RN, Svendsen A, Nielsen JE, Lo Leggio L, Rand KD

R_g^Guinier	3.7	nm
D_max	13.1	nm
Volume^Porod	137	nm³

SASDEG3 – Paenibacillus xanthan lyase (PXL) at 20 °C

Sample:	Paenibacillus xanthan lyase monomer, 113 kDa Paenibacillus sp-62047 protein
Buffer:	20 mM Tris,, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Dec 15

R_g^Guinier	3.8	nm
D_max	13.8	nm
Volume^Porod	134	nm³

SASDS69 – Full length SARS-CoV-2 Non-structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot

Sample:	Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
...Rasmussen A, Pinotsis N, Kozielski F

R_g^Guinier	1.7	nm
D_max	5.9	nm
Volume^Porod	21	nm³

SASDS79 – Long SARS-CoV-2 Non-structural protein 10 (Nsp10: C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) Kratky plot

Sample:	Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

R_g^Guinier	1.6	nm
D_max	5.4	nm
Volume^Porod	19	nm³

SASDS89 – Short SARS-CoV-2 Non-structural protein 10 (Nsp10: N- and C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) Kratky plot

Sample:	Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	16	nm³

SASDS99 – Full length SARS Non-structural protein 10 (Nsp10)

Sample:	Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 6

R_g^Guinier	1.7	nm
D_max	5.9	nm
Volume^Porod	19	nm³

SASDSA9 – Full length MERS Non-structural protein 10 (Nsp10)

Sample:	Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 6

R_g^Guinier	1.7	nm
D_max	5.6	nm
Volume^Porod	19	nm³