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16 hits found for Schirò

SASDUS3 – Dark-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
...Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 4.1 nm
Dmax 14.9 nm
VolumePorod 194 nm3

SASDUT3 – Light-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
...Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 5.0 nm
Dmax 18.5 nm
VolumePorod 170 nm3

SASDNY9 – dark-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.4 nm
Dmax 6.7 nm
VolumePorod 55 nm3

SASDNZ9 – dark-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 77 nm3

SASDP22 – dark-adapted Orange Carotenoid Protein-wtCtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.8 nm
Dmax 12.7 nm
VolumePorod 85 nm3

SASDP32 – dark-adapted Orange Carotenoid Protien-wtCtag at 23 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.8 nm
Dmax 13.0 nm
VolumePorod 89 nm3

SASDP42 – dark-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 44 nm3

SASDP52 – dark-adapted Orange Carotenoid Protein-R27LNtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 61 nm3

SASDP62 – dark-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 58 nm3

SASDP72 – dark-adapted Orange Carotenoid Protein-R27LNtag at 14 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 58 nm3

SASDP82 – light-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 2.9 nm
Dmax 14.0 nm
VolumePorod 63 nm3

SASDP92 – light-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 3.4 nm
Dmax 17.0 nm
VolumePorod 78 nm3

SASDPA2 – light-adapted Orange Carotenoid Protein-wtCtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 4.4 nm
Dmax 31.0 nm
VolumePorod 140 nm3

SASDPB2 – light-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 3.3 nm
Dmax 15.0 nm
VolumePorod 70 nm3

SASDPC2 – light-adapted Orange Carotenoid Protein-R27LNtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 3.4 nm
Dmax 19.0 nm
VolumePorod 70 nm3

SASDPD2 – light-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Schirò G, Colletier JP
RgGuinier 4.8 nm
Dmax 26.0 nm
VolumePorod 120 nm3