Small Angle Scattering Biological Data Bank SASBDB


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9 hits found for Vojtěch Kubáň

SASDB85 – Self-processing module of iron-regulated protein FrpC (415-591)

Iron-regulated protein FrpC (amino acids 415-591) experimental SAS data

Sample:	Iron-regulated protein FrpC (amino acids 415-591) monomer, 19 kDa Neisseria meningitidis protein
Buffer:	5 mM Tris 50 mM NaCl 10 mM CaCl2 0.1% NaN3, pH: 7.4
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2013 Oct 3

Self-processing module of Iron-regulated protein FrpC
Vojtěch Kubáň

R_g^Guinier	1.7	nm
D_max	4.5	nm
Volume^Porod	21	nm³

SASDF58 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data

DNA-directed RNA polymerase subunit delta Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...

R_g^Guinier	3.5	nm
D_max	14.0	nm
Volume^Porod	38	nm³

SASDF68 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	3.9	nm
D_max	20.0	nm
Volume^Porod	56	nm³

SASDF78 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.2	nm
D_max	20.0	nm
Volume^Porod	63	nm³

SASDF88 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 800 mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	21.0	nm
Volume^Porod	77	nm³

SASDF98 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data

DNA-directed RNA polymerase subunit delta - mutant Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.3	nm
D_max	19.5	nm
Volume^Porod	58	nm³

SASDFA8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.6	nm
D_max	22.0	nm
Volume^Porod	76	nm³

SASDFB8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	78	nm³

SASDFC8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	21.0	nm
Volume^Porod	74	nm³

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