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6 hits found for Wenhua Zhang

SASDCN8 – SAXS data of Legionella pneumophila phosphocholine hydrolase Lem3(19-570)

Phosphocholine hydrolase Lem3 experimental SAS data
Phosphocholine hydrolase Lem3 Kratky plot
Sample: Phosphocholine hydrolase Lem3 monomer, 63 kDa Legionella pneumophila subsp. … protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Sep 20
The structural analysis of dephosphocholinase Legionella pneumophila Lem3
Wenhua Zhang
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 95 nm3

SASDCP8 – Ras-related protein Rab-1A

Ras-related protein Rab-1A experimental SAS data
DAMMIF model
Sample: Ras-related protein Rab-1A monomer, 24 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 17
The SAXS Analysis of the human GTPase Rab1a
Wenhua Zhang
RgGuinier 2.4 nm
Dmax 10.2 nm
VolumePorod 36 nm3

SASDET9 – Gliding motility protein MglB: A GAP of Myxococcus xanthus MglA

Gliding motility protein MglB experimental SAS data
GASBOR model
Sample: Gliding motility protein MglB dimer, 34 kDa Myxococcus xanthus protein
Buffer: 150 mM NaCl, 1 mM DTT, 20 mM Tris-HCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 9
MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus. Nat Commun 10(1):5300 (2019)
...Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J
RgGuinier 2.8 nm
Dmax 10.3 nm
VolumePorod 56 nm3

SASDEU9 – Legionella pneumophila Phosphocholinase AnkX

Phosphocholinase AnkX experimental SAS data
GASBOR model
Sample: Phosphocholinase AnkX monomer, 107 kDa Legionella pneumophila protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Oct 20
Legionella pneumophila effector AnkX
Wenhua Zhang
RgGuinier 4.3 nm
Dmax 14.7 nm
VolumePorod 145 nm3

SASDEV9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant

Cytohesin-2; ARNO truncation mutant experimental SAS data
DAMMIN model
Sample: Cytohesin-2; ARNO truncation mutant monomer, 40 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 25
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes. Structure (2019)
...Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
RgGuinier 2.7 nm
Dmax 9.9 nm
VolumePorod 63 nm3

SASDEW9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO

Cytohesin-2 ARF nucleotide-binding site opener experimental SAS data
GASBOR model
Sample: Cytohesin-2 ARF nucleotide-binding site opener dimer, 93 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 23
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes. Structure (2019)
...Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
RgGuinier 4.8 nm
Dmax 19.7 nm
VolumePorod 145 nm3