Small Angle Scattering Biological Data Bank SASBDB


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2 hits found for 147bp 601 Widom sequence

SASDFX3 – Semi-synthetic nucleosome core particle (NCP)

Histone H3Histone H4Histone H2aHistone H2b147bp 601 Widom sequence experimental SAS data

Histone H3 Histone H4 Histone H2a Histone H2b 147bp 601 Widom sequence Kratky plot

Sample:	Histone H3 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 22 kDa Xenopus laevis protein Histone H2a dimer, 28 kDa Xenopus laevis protein Histone H2b dimer, 27 kDa Xenopus laevis protein 147bp 601 Widom sequence monomer, 45 kDa synthetic construct DNA
Buffer:	15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at BM29, ESRF on 2017 Dec 11

A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A

R_g^Guinier	4.4	nm
D_max	12.8	nm
Volume^Porod	353	nm³

SASDFY3 – Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)

Lysyne-specific Demethylase LSD2NPAC linker+DH (delta-205)Histone H3Histone H4Histone H2aHistone H2b147bp 601 Widom sequence experimental SAS data

Lysyne-specific Demethylase LSD2 NPAC linker+DH (delta-205) Histone H3 Histone H4 Histone H2a Histone H2b 147bp 601 Widom sequence Kratky plot

Sample:	Lysyne-specific Demethylase LSD2 monomer, 89 kDa Homo sapiens protein NPAC linker+DH (delta-205) tetramer, 150 kDa Homo sapiens protein Histone H3 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 22 kDa Xenopus laevis protein Histone H2a dimer, 28 kDa Xenopus laevis protein Histone H2b dimer, 27 kDa Xenopus laevis protein 147bp 601 Widom sequence monomer, 45 kDa synthetic construct DNA
Buffer:	15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at BM29, ESRF on 2017 Dec 11

R_g^Guinier	7.8	nm
D_max	30.4	nm
Volume^Porod	1100	nm³

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SASDFX3 – Semi-synthetic nucleosome core particle (NCP)

SASDFY3 – Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)