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8 hits found for Antitoxin ParD

SASDPK3 – ...ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
...antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.2 nm
Dmax 10.0 nm
VolumePorod 140 nm3

SASDPL3 – ...ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
...antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 160 nm3

SASDPM3 – ...ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
...antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.2 nm
Dmax 10.0 nm
VolumePorod 150 nm3

SASDPN3 – ...ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Mar 6
...antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 140 nm3

SASDKY6 – ParD2 antitoxin from Vibrio cholerae in low salt pH 8

Antitoxin ParD experimental SAS data
Antitoxin ParD Kratky plot
Sample: Antitoxin ParD dodecamer, 108 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
...ParD2 antitoxin Acta Crystallographica Section D Structural Biology 77(7):904-920 (2021)
Garcia-Rodriguez G, Girardin Y, Volkov A, Singh R, Muruganandam G, Van Dyck J, Sobott F, Versées W, Charlier D, Loris R
RgGuinier 3.4 nm
Dmax 13.9 nm
VolumePorod 190 nm3

SASDAA9 – EcPaaA2-EcParE2His construct

Plasmid stabilization protein ParE Uncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: Plasmid stabilization protein ParE 16-mer, 188 kDa Escherichia coli protein
...Antitoxin) 16-mer, 135 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 9
...antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 3.8 nm
Dmax 16.2 nm
VolumePorod 312 nm3

SASDAB9 – EcPaaA2-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: Plasmid stabilization protein ParE octamer, 102 kDa Escherichia coli protein
...Antitoxin) octamer, 68 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
...antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 3.3 nm
Dmax 15.3 nm
VolumePorod 166 nm3

SASDC84 – parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein experimental SAS data
parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct Rg histogram
Sample: Plasmid stabilization protein ParE monomer, 13 kDa Escherichia coli protein
Uncharacterized protein monomer, 6 kDa Escherichia coli O157:H7 protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
...antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 2.2 nm
Dmax 9.3 nm
VolumePorod 36 nm3