| MWI(0) | 23 | kDa |
| MWexpected | 17 | kDa |
| VPorod | 57 | nm3 |
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log I(s)
7.20×10-3
7.20×10-4
7.20×10-5
7.20×10-6
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s, nm-1
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Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region.
Mukhopadhyay S, Subedi S, Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC, Autophagy :1-17 (2023) Europe PMCSASDQY9 – Beclin-1 amino acids 1-150 with cysteine motif residues mutated to serine, along with a C-terminal tyrosine
Beclin-1 (C18S, C21S, A103V, C137S, C140S)|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of Beclin-1 amino acids 1-150 with cysteine motif residues mutated to serine in 50 mM Tris, 300 mM NaCl, pH 8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 22°C. 1233 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The experimental molecular weight was determined using SEC-MALS. |
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