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19 hits found for ColH protein

SASDC44 – Envelope of Col H PKD-CBD complexed with mini-collagen

ColH proteinCollagenous Peptide model [(PPG)10] experimental SAS data
DAMMIF model
Sample: ColH protein monomer, 24 kDa Hathewaya histolytica protein
Collagenous Peptide model [(PPG)10] trimer, 9 kDa synthetic construct protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ -induced orientation of tandem collagen binding domains from clostridial collagenase ColG permits two opposing functions of collagen fibril formation and retardation. FEBS J 285(17):3254-3269 (2018)
Caviness P, Bauer R, Tanaka K, Janowska K, Roeser JR, Harter D, Sanders J, Ruth C, Matsushita O, Sakon J
RgGuinier 2.7 nm
Dmax 12.0 nm
VolumePorod 28 nm3

SASDC54 – Envelope of Col H PKD-PKD-CBD complexed with mini-collagen

ColH proteinCollagenous Peptide model [(PPG)10] experimental SAS data
DAMMIF model
Sample: ColH protein monomer, 34 kDa Hathewaya histolytica protein
Collagenous Peptide model [(PPG)10] trimer, 10 kDa synthetic construct protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ -induced orientation of tandem collagen binding domains from clostridial collagenase ColG permits two opposing functions of collagen fibril formation and retardation. FEBS J 285(17):3254-3269 (2018)
Caviness P, Bauer R, Tanaka K, Janowska K, Roeser JR, Harter D, Sanders J, Ruth C, Matsushita O, Sakon J
RgGuinier 3.3 nm
Dmax 14.2 nm
VolumePorod 38 nm3

SASDJQ6 – Full-length Collagenase H containing N-terminal Catalytic domain and C-terminal Non-Catalytic domains Polycystic Kidney disease domain 1 (PKD1), Polycystic Kidney disease domain 2 (PKD2) and Collagen ...

Collagenase ColH (Full-length) experimental SAS data
DAMFILT model
Sample: ...ColH (Full-length) monomer, 112 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES, 100 mM NaCl, 0.4 mM EGTA, 2.4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 12
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 198 nm3

SASDJR6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)

Collagenase ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) experimental SAS data
DAMFILT model
Sample: ...ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) monomer, 23 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES, 100 mM NaCl, 0.4 mM EGTA, 2.4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 25
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 2.6 nm
Dmax 8.7 nm
VolumePorod 29 nm3

SASDJS6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) bound to triple helical collagen like-peptide [(POG)10]3

Collagenous Peptide model [(PPG)10]Collagenase ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) experimental SAS data
DAMFILT model
Sample: Collagenous Peptide model [(PPG)10] trimer, 9 kDa synthetic construct protein
...ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) monomer, 23 kDa Hathewaya histolytica protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 12
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 3.5 nm
Dmax 14.5 nm
VolumePorod 41 nm3

SASDJT6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1, Polycystic Kidney disease domain 2 and Collagen binding domain (CBD) with Y689S, F712S, Y780S, H782S, Y796S and Y801...

Collagen like-peptide [GPRG(POG)13]Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801) experimental SAS data
DAMFILT model
Sample: Collagen like-peptide [GPRG(POG)13] trimer, 11 kDa protein
...ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801) monomer, 33 kDa Hathewaya histolytica protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 4.0 nm
Dmax 22.0 nm
VolumePorod 61 nm3

SASDJU6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1 (PKD1), Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780...

Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801) experimental SAS data
DAMFILT model
Sample: ...ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801) monomer, 33 kDa Hathewaya histolytica protein
Buffer: 50 mM Tris, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 3.3 nm
Dmax 11.0 nm
VolumePorod 34 nm3

SASDJV6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1 (PKD1), Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser and Phe712Ser boun...

Collagen like-peptide [GPRG(POG)13]Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser and Phe712Ser) experimental SAS data
DAMFILT model
Sample: Collagen like-peptide [GPRG(POG)13] trimer, 11 kDa protein
...ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser and Phe712Ser) monomer, 33 kDa Hathewaya histolytica protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 12
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 4.4 nm
Dmax 21.0 nm
VolumePorod 79 nm3

SASDJW6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1 (PKD1), Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser and Phe712Ser

Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser and Phe712Ser) experimental SAS data
DAMFILT model
Sample: ...ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser and Phe712Ser) monomer, 33 kDa Hathewaya histolytica protein
Buffer: 50 mM Tris, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 3.7 nm
Dmax 11.5 nm
VolumePorod 38 nm3

SASDJX6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease 1 (PKD1) domain, Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) bound to triple helical collagen ...

Collagen like-peptide [GPRG(POG)13]Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) experimental SAS data
DAMFILT model
Sample: Collagen like-peptide [GPRG(POG)13] trimer, 11 kDa protein
...ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) monomer, 34 kDa Hathewaya histolytica protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 4.0 nm
Dmax 22.0 nm
VolumePorod 63 nm3

SASDJY6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1 (PKD1), Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)

Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) experimental SAS data
DAMFILT model
Sample: ...ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) monomer, 34 kDa Hathewaya histolytica protein
Buffer: 10 mM HEPES, 100 mM NaCl, 0.4 mM EGTA, 2.4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 32 nm3

SASDJZ6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr780Ser, His782Ser, Tyr796Ser, Tyr801Ser

Collagenase ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr780Ser, His782Ser, Tyr796Ser and Tyr801Ser) experimental SAS data
DAMFILT model
Sample: ...ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr780Ser, His782Ser, Tyr796Ser and Tyr801Ser) monomer, 23 kDa Hathewaya histolytica protein
Buffer: 50 mM Tris, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 20 nm3

SASDJ27 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr780Ser, His782Ser, Tyr796Ser and Tyr801Ser bound to triple helical c...

Collagen like-peptide [GPRG(POG)13]Collagenase ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr780Ser, His782Ser, Tyr796Ser and Tyr801Ser) experimental SAS data
DAMFILT model
Sample: Collagen like-peptide [GPRG(POG)13] trimer, 11 kDa protein
...ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr780Ser, His782Ser, Tyr796Ser and Tyr801Ser) monomer, 23 kDa Hathewaya histolytica protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 3.2 nm
Dmax 18.0 nm
VolumePorod 34 nm3

SASDJ37 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) bound to triple helical collagen like-peptide

Collagenase ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD))Collagen like-peptide [GPRG(POG)13] experimental SAS data
DAMFILT model
Sample: ...ColH (Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD)) monomer, 23 kDa Hathewaya histolytica protein
Collagen like-peptide [GPRG(POG)13] trimer, 11 kDa protein
Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 5
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in... University of Arkansas PhD thesis 28030553 (2020)
Perry Caviness
RgGuinier 3.3 nm
Dmax 18.0 nm
VolumePorod 35 nm3

SASDCN4 – ...ColH s2as2bs3 at pCa 3

Collagenase ColH segement s2as2bs3 experimental SAS data
DAMMIF model
Sample: ...ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
...ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.3 nm
Dmax 13.6 nm
VolumePorod 35 nm3

SASDCP4 – ...ColH s2as2bs3 at pCa 4

Collagenase ColH segement s2as2bs3 experimental SAS data
DAMMIF model
Sample: ...ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
...ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.2 nm
Dmax 13.1 nm
VolumePorod 37 nm3

SASDCQ4 – ...ColH s2as2bs3 at pCa 5

Collagenase ColH segement s2as2bs3 experimental SAS data
DAMMIF model
Sample: ...ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
...ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.3 nm
Dmax 14.2 nm
VolumePorod 37 nm3

SASDCR4 – ...ColH s2as2bs3 at pCa 6

Collagenase ColH segement s2as2bs3 experimental SAS data
DAMMIF model
Sample: ...ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
...ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.2 nm
Dmax 15.5 nm
VolumePorod 35 nm3

SASDC35 – ...ColH s2as2bs3 at pCa 7

Collagenase ColH segement s2as2bs3 experimental SAS data
DAMMIF model
Sample: ...ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
...ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.0 nm
Dmax 14.4 nm
VolumePorod 40 nm3