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Sample: |
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex monomer, 4 kDa Escherichia coli protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
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Buffer: |
PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
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Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Nov 8
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...of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
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RgGuinier |
2.3 |
nm |
Dmax |
10.7 |
nm |
VolumePorod |
13 |
nm3 |
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