Dystrophin's central domain forms a complex filament that becomes disorganized by in-frame deletions.

Delalande O, Molza AE, Dos Santos Morais R, Chéron A, Pollet É, Raguenes-Nicol C, Tascon C, Giudice E, Guilbaud M, Nicolas A, Bondon A, Leturcq F, Férey N, Baaden M, Perez J, Roblin P, Piétri-Rouxel F, Hubert JF, Czjzek M, Le Rumeur E, J Biol Chem 293(18):6637-6646 (2018) Europe PMC

SASDBA3 – Human dystrophin central domain repeats 20 to 24

Dystrophin central domain repeats 20 to 24.
MWexperimental 67 kDa
MWexpected 67 kDa
VPorod 107 nm3
log I(s) 4.00×10-4 4.00×10-5 4.00×10-6 4.00×10-7
Dystrophin central domain repeats 20 to 24. small angle scattering data  s, nm-1
ln I(s)
Dystrophin central domain repeats 20 to 24. Guinier plot ln 4×10-4 Rg: 5.8 nm 0 (5.8 nm)-2 s2
(sRg)2I(s)/I(0)
Dystrophin central domain repeats 20 to 24. Kratky plot 1.104 0 √3 sRg
p(r)
Dystrophin central domain repeats 20 to 24. pair distance distribution function Rg: 6.1 nm 0 Dmax: 22.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Dystrophin central domain repeats 20 to 24. NONE model
Synchrotron SAXS data were measured from solutions of Human Dystrophin central domain repeats 20 to 24 (amino acids 2469-3040) in 20 mM Tris 150 mM NaCl, 1 mM EDTA, 2% glycerol buffer using size exclusion chromatography (SEC-SAXS) in combination with UV spectroscopy on the SWING beam line (SOLEIL, Saint-Aubin, France). The sample load concentration was 20.5 mg/mL. The scattering data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected through the corresponding SEC protein elution peak (20 x 1.5 s frames) as well as from the SEC column running buffer. The buffer scattering contributions were subtracted from each sample frame, which were then scaled and averaged to produce the SAXS profile displayed in this entry. The molecular weight estimate (MW) are derived from the Porod volume, Vp, where MW is approximately Vp/1.6. The SEC Rg and I(0) and UV trace are included in the additional files to this entry.

Cell temperature = UNKNOWN. Concentration = UNKNOWN

Dystrophin central domain repeats 20 to 24. (Dystrophin 2469-3040)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   66.7 kDa
 
UniProt   P11532 (2469-3040)
Sequence   FASTA