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5 hits found for Estrogen receptor

SASDEE2 – ...estrogen receptor alpha

Estrogen receptor experimental SAS data
CUSTOM IN-HOUSE model
Sample: Estrogen receptor monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2017 Jun 12
...Estrogen Receptor Transactivation Domain. Structure 27(2):229-240.e4 (2019)
Peng Y, Cao S, Kiselar J, Xiao X, Du Z, Hsieh A, Ko S, Chen Y, Agrawal P, Zheng W, Shi W, Jiang W, Yang L, Chance MR, Surewicz WK, Buck M, Yang S
RgGuinier 3.0 nm
Dmax 10.0 nm

SASDMZ4 – ...estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml

Estrogen-related receptor gammaInverse repeat IR3 DNA experimental SAS data
SASREF MX model
Sample: Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein
Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3
Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules Biochemistry 52(39):6844-6855 (2013)
Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D
RgGuinier 4.7 nm
Dmax 14.6 nm

SASDM25 – ...estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.9 mg/ml

Estrogen-related receptor gammaInverse repeat IR3 DNA experimental SAS data
SASREF MX model
Sample: Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein
Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3
Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules Biochemistry 52(39):6844-6855 (2013)
Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D
RgGuinier 4.3 nm
Dmax 13.2 nm

SASDM35 – ...estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.0 mg/ml

Estrogen-related receptor gammaInverse repeat IR3 DNA experimental SAS data
SASREF MX model
Sample: Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein
Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3
Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules Biochemistry 52(39):6844-6855 (2013)
Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D
RgGuinier 4.1 nm
Dmax 12.8 nm

SASDDU8 – ...estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains

Estrogen receptorERE1ERE2EstradiolhERa peptide1hERa peptide2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Estrogen receptor dimer, 85 kDa protein
ERE1 monomer, 6 kDa Homo sapiens DNA
ERE2 monomer, 6 kDa Homo sapiens DNA
Estradiol dimer, 0 kDa
hERa peptide1 monomer, 2 kDa protein
hERa peptide2 monomer, 2 kDa protein
Buffer: 10 mM CHES (pH9.5), 125 mM NaCl, 5mM KCl, 4 mM MgCl2, 50 mM arginine, 50 mM glutamate, 5 mM TCEP, 5% glycerol, 10 µm Zn acetate, 10 µM estradiol, pH: 9.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Aug 10
...estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains. Nat Commun 9(1):3520 (2018)
Huang W, Peng Y, Kiselar J, Zhao X, Albaqami A, Mendez D, Chen Y, Chakravarthy S, Gupta S, Ralston C, Kao HY, Chance MR, Yang S
RgGuinier 3.8 nm
Dmax 11.5 nm