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5 hits found for Eukaryotic initiation factor 4A-I

SASDQT6 – ...eukaryotic initiation factor 4A1

Eukaryotic initiation factor 4A-I experimental SAS data
Eukaryotic initiation factor 4A-I Kratky plot
Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 22
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 3.1 nm
Dmax 9.8 nm
VolumePorod 80 nm3

SASDQU6 – eukaryotic initiation factor 4A1 bound to 20 nt (AG)10 ssRNA

Eukaryotic initiation factor 4A-I(AG)10-RNA experimental SAS data
Eukaryotic initiation factor 4A-I (AG)10-RNA Kratky plot
Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein
(AG)10-RNA monomer, 7 kDa synthetic construct RNA
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 96 nm3

SASDQV6 – eukaryotic initiation factor 4A1 bound to 20 nt (CAA)6CA ssRNA

Eukaryotic initiation factor 4A-I(CAA)6CA-RNA experimental SAS data
Eukaryotic initiation factor 4A-I (CAA)6CA-RNA Kratky plot
Sample: Eukaryotic initiation factor 4A-I monomer, 46 kDa Homo sapiens protein
(CAA)6CA-RNA monomer, 6 kDa synthetic construct RNA
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 22
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 3.1 nm
Dmax 12.8 nm
VolumePorod 89 nm3

SASDQW6 – ...eukaryotic initiation factor 4A1 bound to 20 nt (AG)10 ssRNA

(AG)10-RNAEukaryotic initiation factor 4A-I experimental SAS data
(AG)10-RNA Eukaryotic initiation factor 4A-I Kratky plot
Sample: (AG)10-RNA monomer, 7 kDa synthetic construct RNA
Eukaryotic initiation factor 4A-I trimer, 138 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 3.4 nm
Dmax 13.1 nm
VolumePorod 150 nm3

SASDQX6 – ...eukaryotic initiation factor 4A1 bound to 20 nt (AG)10-24bp duplex RNA

Eukaryotic initiation factor 4A-I(AG)10-24bp loading-RNA(AG)10-24bp duplex-RNA experimental SAS data
Eukaryotic initiation factor 4A-I (AG)10-24bp loading-RNA (AG)10-24bp duplex-RNA Kratky plot
Sample: Eukaryotic initiation factor 4A-I trimer, 138 kDa Homo sapiens protein
(AG)10-24bp loading-RNA monomer, 15 kDa synthetic construct RNA
(AG)10-24bp duplex-RNA monomer, 7 kDa synthetic construct RNA
Buffer: 20 mM Hepes, 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 16
eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res (2023)
Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, Scott D, Carlin LM, Huang D, Le Quesne J, Zanivan S, Wilczynska A, Bushell M
RgGuinier 4.9 nm
Dmax 16.2 nm
VolumePorod 230 nm3