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4 hits found for Glutamate--tRNA ligase

SASDLY5 – Monomer-dimer equilibrium of glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glutamate--tRNA ligase dimer, 108 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 35 mM HEPES⁄NaOH, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 19
Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl-tRNA synthetase and determination of its oligomeric structure in solution FEBS Journal 276(5):1398-1417 (2009)
Paravisi S, Fumagalli G, Riva M, Morandi P, Morosi R, Konarev P, Petoukhov M, Bernier S, Chênevert R, Svergun D, Curti B, Vanoni M
RgGuinier 3.5 nm
Dmax 11.0 nm
VolumePorod 123 nm3

SASDQ37 – N-terminal GST-like domain of Plasmodium berghei cytosolic glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data
OLIGOMER model
Sample: Glutamate--tRNA ligase dimer, 57 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDQ67 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, Q-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and glutaminyl-tRNA synthetase

Glutamine--tRNA ligaseGlutamate--tRNA ligasetRNA import protein tRIP experimental SAS data
CORAL model
Sample: ...ligase dimer, 46 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
tRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.6 nm
Dmax 17.1 nm
VolumePorod 198 nm3

SASDQ77 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase

Methionine--tRNA ligasetRNA import protein tRIPGlutamate--tRNA ligase experimental SAS data
CORAL model
Sample: ...ligase dimer, 55 kDa Plasmodium berghei (strain … protein
tRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.7 nm
Dmax 18.3 nm
VolumePorod 217 nm3