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4 hits found for HpcH/HpaI aldolase

SASDKS7 – ...aldolase (HpcH/HpaI aldolase), wild-type in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer: 20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Substrate Induced Movement of the Metal Cofactor between Active and Resting State Angewandte Chemie International Edition (2022)
Marsden S, Wijma H, Mohr M, Justo I, Hagedoorn P, Laustsen J, Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U
RgGuinier 3.3 nm
Dmax 9.0 nm
VolumePorod 224 nm3

SASDKT7 – ...aldolase (HpcH/HpaI aldolase), S116A mutant in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase (S116A) experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase (S116A) hexamer, 165 kDa Sphingomonas wittichii (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Substrate Induced Movement of the Metal Cofactor between Active and Resting State Angewandte Chemie International Edition (2022)
Marsden S, Wijma H, Mohr M, Justo I, Hagedoorn P, Laustsen J, Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U
RgGuinier 3.3 nm
Dmax 9.1 nm
VolumePorod 222 nm3

SASDKU7 – ...aldolase (HpcH/HpaI aldolase), S116C mutant in HEPES, NaCl and MgCl2

HpcH/HpaI aldolase (S116C) experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase (S116C) hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer: 20 mM HEPES, 100 mM NaCl, 20 mg/l MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Substrate Induced Movement of the Metal Cofactor between Active and Resting State Angewandte Chemie International Edition (2022)
Marsden S, Wijma H, Mohr M, Justo I, Hagedoorn P, Laustsen J, Jeffries C, Svergun D, Mestrom L, McMillan D, Bento I, Hanefeld U
RgGuinier 3.3 nm
Dmax 9.0 nm
VolumePorod 223 nm3

SASDEX9 – ...aldolase (HpcH/HpaI aldolase), wild-type hexamer in HEPES isolated using SEC-SAXS

HpcH/HpaI aldolase experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer: 20 mM HEPES,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
EFAMIX , a tool to decompose inline chromatography SAXS data from partially overlapping components Protein Science (2021)
Konarev P, Graewert M, Jeffries C, Fukuda M, Cheremnykh T, Volkov V, Svergun D
RgGuinier 3.3 nm
Dmax 9.4 nm
VolumePorod 233 nm3