Small Angle Scattering Biological Data Bank SASBDB


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6 hits found for Nicked DNA

SASDFH6 – ...DNA ligase-A tricomplex in the presence of DNA substrate

Probable exodeoxyribonuclease III protein XthADNA ligase ABeta sliding clampDNA ligase A nicked DNA substrate experimental SAS data

Sample:	Probable exodeoxyribonuclease III protein XthA monomer, 31 kDa Mycobacterium tuberculosis protein DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein DNA ligase A nicked DNA substrate dimer, 16 kDa DNA
Buffer:	50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 17

Regulation of futile ligation during early steps of BER in M. tuberculosis is carried out by a β-clamp-XthA-LigA tri-component complex International Journal of Biological Macromolecules (2022)
Shukla A, Afsar M, Khanam T, Kumar N, Ali F, Kumar S, Jahan F, Ramachandran R

R_g^Guinier	5.8	nm
D_max	19.1	nm
Volume^Porod	496	nm³

SASDEU5 – ...DNA ligase A

Sample:	DNA ligase A monomer, 38 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Sep 27

...DNA ligase A (MtbLigA) are important for the initial steps of nick-sealing activity Acta Crystallographica Section D Structural Biology 77(6) (2021)
Afsar M, Shukla A, Kumar N, Ramachandran R

R_g^Guinier	2.4	nm
D_max	8.8	nm
Volume^Porod	69	nm³

SASDEC6 – ...DNA ligase A with NAD+

Sample:	DNA ligase A monomer, 38 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Oct 6

R_g^Guinier	2.4	nm
D_max	6.2	nm
Volume^Porod	94	nm³

SASDG65 – ...DNA ligase A (LigA)

Sample:	DNA ligase A monomer, 37 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl 200 mM NaCl 2mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 May 21

R_g^Guinier	2.7	nm
D_max	8.9	nm
Volume^Porod	68	nm³

SASDDV9 – ...DNA LigaseA with Nicked DNA

DNA ligase ANicked DNA experimental SAS data

Sample:	DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Nicked DNA dimer, 16 kDa DNA
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 13

...DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. Nucleic Acids Res (2020)
Khanam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R

R_g^Guinier	4.4	nm
D_max	14.8	nm
Volume^Porod	262	nm³

SASDEX3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA

DNA ligase AProbable exodeoxyribonuclease III protein XthANicked DNA experimental SAS data

Sample:	DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein Nicked DNA monomer, 8 kDa DNA
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 13