Small Angle Scattering Biological Data Bank SASBDB


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12 hits found for Nucleoplasmin

SASDBY4 – ...Nucleoplasmin-histone H2A/H2B complex

Nucleoplasmin core + A2Histone H2A (ΔAla127)Histone H2B 1.1 (Ser33Thr) experimental SAS data

Sample:	Nucleoplasmin core + A2 pentamer, 81 kDa Xenopus laevis protein Histone H2A (ΔAla127) pentamer, 69 kDa Xenopus laevis protein Histone H2B 1.1 (Ser33Thr) pentamer, 67 kDa Xenopus laevis protein
Buffer:	20 mM Tris. 150 mM NaCl, 1 mM EDTA, 5 mM DTT, pH: 8
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2016 Jan 7

...nucleoplasmin controls histone binding and release. Nat Commun 8(1):2215 (2017)
Warren C, Matsui T, Karp JM, Onikubo T, Cahill S, Brenowitz M, Cowburn D, Girvin M, Shechter D

R_g^Guinier	4.4	nm
D_max	14.0	nm
Volume^Porod	402	nm³

SASDA55 – Nucleoplasmin

Sample:	Nucleoplasmin pentamer, 110 kDa Escherichia coli protein
Buffer:	20 mM Pipes buffer 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 3

...nucleoplasmin: thermodynamic and structural models. J Mol Biol 393(2):448-63 (2009)
Taneva SG, Bañuelos S, Falces J, Arregi I, Muga A, Konarev PV, Svergun DI, Velázquez-Campoy A, Urbaneja MA

R_g^Guinier	4.0	nm
D_max	12.6	nm
Volume^Porod	210	nm³

SASDA65 – Nucleoplasmin-H5 complex

Sample:	NP-H5 pentamer, 200 kDa Escherichia coli protein
Buffer:	20 mM Pipes buffer 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 3

R_g^Guinier	5.2	nm
D_max	16.7	nm
Volume^Porod	410	nm³

SASDA75 – Nucleoplasmin-H2AH2B complex

Sample:	NP-H2AH2B pentamer, 250 kDa Escherichia coli protein
Buffer:	20 mM Pipes buffer 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2014 Dec 3

R_g^Guinier	4.7	nm
D_max	14.5	nm
Volume^Porod	470	nm³

SASDAC5 – ImportinA_ImportinB

ImportinA_ImportinB experimental SAS data

Sample:	ImportinA_ImportinB monomer, 160 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Oct 29

...nucleoplasmin by its nuclear transport receptor importin α/β: insights into a complete import complex. Biochemistry 49(45):9756-69 (2010)
Falces J, Arregi I, Konarev PV, Urbaneja MA, Svergun DI, Taneva SG, Bañuelos S

R_g^Guinier	5.7	nm
D_max	19.0	nm
Volume^Porod	390	nm³

SASDAD5 – Nucleoplasmin_ImpA_ImpB

Nucleoplasmin_importinA_importinB experimental SAS data

Sample:	Nucleoplasmin_importinA_importinB , 900 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2008 May 23

R_g^Guinier	8.6	nm
D_max	28.0	nm
Volume^Porod	1800	nm³

SASDB47 – Full length tetrameric FKBP39 protein from Drosophila melanogaster

39 kDa FK506-binding nuclear protein experimental SAS data

Full length tetrameric FKBP39 protein from Drosophila melanogaster Rg histogram

Sample:	39 kDa FK506-binding nuclear protein tetramer, 163 kDa Drosophila melanogaster protein
Buffer:	50 mM Na2HPO4 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2014 Nov 14

Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments. Sci Rep 7:40405 (2017)
Kozłowska M, Tarczewska A, Jakób M, Bystranowska D, Taube M, Kozak M, Czarnocki-Cieciura M, Dziembowski A, Orłowski M, Tkocz K, Ożyhar A

R_g^Guinier	5.7	nm
D_max	22.0	nm
Volume^Porod	275	nm³