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2 hits found for Phosphoenolpyruvate-protein phosphotransferase

SASDCN3 – Phosphoenolpyruvate-protein phosphotransferase

Phosphoenolpyruvate-protein phosphotransferase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Phosphoenolpyruvate-protein phosphotransferase dimer, 127 kDa Escherichia coli protein
Buffer: 20mM TRIS buffer, 100 mM NaCl, 10 mM DTT, 4 mM MgCl2, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at 12-ID-C, Advanced Photon Source (APS), Argonne National Laboratory on 2010 Aug 23
Solution structure of the 128 kDa enzyme I dimer from Escherichia coli and its 146 kDa complex with HPr using residual dipolar couplings and small- and wide-angle X-ray scattering. J Am Chem Soc 132(37):13026-45 (2010)
Schwieters CD, Suh JY, Grishaev A, Ghirlando R, Takayama Y, Clore GM
RgGuinier 4.1 nm
Dmax 14.8 nm
VolumePorod 189 nm3

SASDBZ5 – Enzyme I-Ntr (residues 170-424) in complex with NPr (residues 1-85)

Phosphocarrier protein NPrPhosphoenolpyruvate-protein phosphotransferase PtsP experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Phosphocarrier protein NPr monomer, 9 kDa Escherichia coli protein
Phosphoenolpyruvate-protein phosphotransferase PtsP monomer, 28 kDa Escherichia coli protein
Buffer: 10 mM Tris 150 mM NaCl 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, NIDDK, NIH on 2015 Jan 30
...Phosphotransferase Systems. Structure 24(12):2127-2137 (2016)
Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 52 nm3