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23 hits found for Proline utilization A

...ASDDL2 – ...Proline Utilization A (PutA) lowest concentration, 1.00 mg/ml

Sinorhizobium meliloti (SmPutA) experimental SAS data
MES-FOXS model
Sample: ...A) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer: ...and 50 mM NaCl, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...ar 27
...Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function. J Biol Chem 291(46):24065-24075 (2016)
...amage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 171 nm3

...ASDDM2 – ...Proline Utilization A (PutA) at 2.00 mg/ml

Sinorhizobium meliloti (SmPutA) experimental SAS data
MES-FOXS model
Sample: ...A) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer: ...and 50 mM NaCl, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...ar 27
...Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function. J Biol Chem 291(46):24065-24075 (2016)
...amage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ
RgGuinier 3.8 nm
Dmax 11.9 nm
VolumePorod 225 nm3

...ASDDN2 – ...Proline Utilization A (PutA) at 3.00 mg/ml

Sinorhizobium meliloti (SmPutA) experimental SAS data
MES-FOXS model
Sample: ...A) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer: ...and 50 mM NaCl, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...ar 27
...Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function. J Biol Chem 291(46):24065-24075 (2016)
...amage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ
RgGuinier 3.8 nm
Dmax 11.8 nm
VolumePorod 248 nm3

...ASDDP2 – ...Proline Utilization A (PutA) at high concentration, 4.00 mg/ml

Sinorhizobium meliloti (SmPutA) experimental SAS data
MES-FOXS model
Sample: ...A) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer: ...and 50 mM NaCl, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...ar 27
...Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function. J Biol Chem 291(46):24065-24075 (2016)
...amage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ
RgGuinier 3.9 nm
Dmax 11.9 nm
VolumePorod 277 nm3

...ASDCP3 – Proline utilization A from Bdellovibrio bacteriovorus

Bifunctional protein PutA experimental SAS data
DAMMIF model
Sample: ...al protein PutA dimer, 219 kDa ...acteriovorus protein
Buffer: ...aCl, 1 mM EDTA, and 1 mM tris(3-hydroxypropyl)phosphine (THP) at pH 7.5,, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.5 nm
Dmax 14.0 nm
VolumePorod 287 nm3

...ASDDP3 – ...argyl glycine-Inactivated Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) collected by SEC-SAXS

Bifunctional protein PutA experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...al protein PutA dimer, 215 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM TCEP, 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at ...AT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jul 16
...ation of Oligomeric State in Proline Utilization A. Biophys J 114(12):2833-2843 (2018)
...asick DA, Campbell AC, Christgen SL, Chakravarthy S, White TA, Becker DF, Tanner JJ
RgGuinier 4.6 nm
Dmax 14.4 nm
VolumePorod 324 nm3

...ASDCQ3 – Proline utilization A from Desulfovibrio vulgaris 1.5 mg/mL

Bifunctional protein PutA experimental SAS data
Bifunctional protein PutA Kratky plot
Sample: ...al protein PutA dimer, 229 kDa ...aris protein
Buffer: ...aCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.4 nm
Dmax 16.0 nm
VolumePorod 293 nm3

...ASDDQ3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) collected by SEC-SAXS

Bifunctional protein PutA experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...al protein PutA ...amer, 430 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM TCEP, 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at ...AT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jul 16
...ation of Oligomeric State in Proline Utilization A. Biophys J 114(12):2833-2843 (2018)
...asick DA, Campbell AC, Christgen SL, Chakravarthy S, White TA, Becker DF, Tanner JJ
RgGuinier 5.2 nm
Dmax 14.2 nm
VolumePorod 582 nm3

...ASDCR3 – Proline utilization A from Legionella pneumophila 3 mg/mL

Bifunctional protein PutA experimental SAS data
Bifunctional protein PutA Kratky plot
Sample: ...al protein PutA dimer, 238 kDa ...a pneumophila subsp. … protein
Buffer: ...aCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...Apr 20
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 291 nm3

...ASDCS3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) 2.3 mg/mL

Proline dehydrogenase experimental SAS data
Proline dehydrogenase Kratky plot
Sample: Proline dehydrogenase ...amer, 430 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 5.3 nm
Dmax 14.1 nm
VolumePorod 541 nm3

...ASDCT3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) 4.7 mg/mL

Proline dehydrogenase experimental SAS data
Proline dehydrogenase Kratky plot
Sample: Proline dehydrogenase ...amer, 430 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 12
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 5.2 nm
Dmax 14.6 nm
VolumePorod 553 nm3

...ASDCU3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) 7.0 mg/mL

Proline dehydrogenase experimental SAS data
DAMMIF model
Sample: Proline dehydrogenase ...amer, 430 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 12
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 5.2 nm
Dmax 13.7 nm
VolumePorod 560 nm3

...ASDCV3 – Proline utilization A from Legionella pneumophila 5 mg/mL

Bifunctional protein PutA experimental SAS data
Bifunctional protein PutA Kratky plot
Sample: ...al protein PutA dimer, 238 kDa ...a pneumophila subsp. … protein
Buffer: ...aCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...Apr 20
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.6 nm
Dmax 15.3 nm
VolumePorod 297 nm3

...ASDCW3 – Proline utilization A from Legionella pneumophila 8 mg/mL

Bifunctional protein PutA experimental SAS data
DAMMIF model
Sample: ...al protein PutA dimer, 238 kDa ...a pneumophila subsp. … protein
Buffer: ...aCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...Apr 20
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.6 nm
Dmax 15.5 nm
VolumePorod 295 nm3

...ASDCX3 – Proline utilization A from Desulfovibrio vulgaris 3.0 mg/mL

Bifunctional protein PutA experimental SAS data
DAMMIF model
Sample: ...al protein PutA dimer, 229 kDa ...aris protein
Buffer: ...aCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.4 nm
Dmax 16.0 nm
VolumePorod 295 nm3

...ASDCY3 – Proline utilization A from Desulfovibrio vulgaris 4.5 mg/mL

Bifunctional protein PutA experimental SAS data
Bifunctional protein PutA Kratky plot
Sample: ...al protein PutA dimer, 229 kDa ...aris protein
Buffer: ...aCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5., pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Jun 8
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.4 nm
Dmax 16.0 nm
VolumePorod 294 nm3

...ASDCZ3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 2.3 mg/mL

Proline dehydrogenase experimental SAS data
Proline dehydrogenase Kratky plot
Sample: Proline dehydrogenase dimer, 215 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.5 nm
Dmax 14.5 nm
VolumePorod 281 nm3

...ASDC24 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 4.7 mg/mL

Proline dehydrogenase experimental SAS data
Proline dehydrogenase Kratky plot
Sample: Proline dehydrogenase dimer, 215 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.5 nm
Dmax 13.9 nm
VolumePorod 283 nm3

...ASDC34 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 7.0 mg/mL

Proline dehydrogenase experimental SAS data
DAMMIF model
Sample: Proline dehydrogenase dimer, 215 kDa ...adyrhizobium diazoefficiens protein
Buffer: ...aCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH: 7.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 16
...al investigation of type A PutAs reveals a conserved core oligomeric structure. FEBS J 284(18):3029-3049 (2017)
...asick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
RgGuinier 4.5 nm
Dmax 14.6 nm
VolumePorod 289 nm3

...ASDB27 – ...A: The E.coli Proline utilization A RHH domain fused to R.capsulatus PutA

Proline utilization A experimental SAS data
Proline utilization A Kratky plot
Sample: Proline utilization A dimer, 251 kDa ...a coli, Rhodobacter … protein
Buffer: ...aCl, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Oct 16
...a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA. Biosci Rep 36(6) (2016)
Arentson BW, Hayes EL, Zhu W, Singh H, Tanner JJ, Becker DF
RgGuinier 5.2 nm
Dmax 18.3 nm
VolumePorod 308 nm3

...ASDQ48 – ...al proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 1.1 mg/mL

Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: ...al proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: ...aCl, and 1mM TCEP, pH: 7.6
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...Apr 12
...ased engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
...AN, Ji J, Tanner JJ
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 102 nm3

...ASDQ58 – ...al proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 2.3 mg/mL

Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: ...al proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: ...aCl, and 1mM TCEP, pH: 7.6
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...Apr 12
...ased engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
...AN, Ji J, Tanner JJ
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 102 nm3

...ASDQ68 – ...al proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 3.4 mg/mL

Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: ...al proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: ...aCl, and 1mM TCEP, pH: 7.6
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...Apr 12
...ased engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
...AN, Ji J, Tanner JJ
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 108 nm3