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5 hits found for Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant)

SASDSP5 – ...H163A Mutant - 0.25 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
...H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 63 nm3

SASDSQ5 – ...H163A Mutant - 0.5 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
...H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 74 nm3

SASDSR5 – ...H163A Mutant - 1.0 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
...H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 8.2 nm
VolumePorod 85 nm3

SASDSS5 – ...H163A Mutant - 3.0 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
...H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 102 nm3

SASDST5 – ...H163A Mutant - 6.3 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
...H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 7.7 nm
VolumePorod 98 nm3